Registration Dossier
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EC number: 250-480-2 | CAS number: 31138-65-5
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
An algal growth study was conducted on the test material, this study was conducted according to OECD guideline 201 and EU Method C3. The results of this study showed Sodium Glucoheptonate to have an EC50 value of 790 mg/L and a NOEC of 100 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 790 mg/L
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
Introduction:
A study was performed to assess the effect of the test item on the growth of the green alga Pseudokirchneriella subcapitata. The method followed that described in the OECD Guidelines for Testing of Chemicals (2006) No 201, "Freshwater Alga and Cyanobacteria, Growth Inhibition Test" referenced as Method C.3 of Commission Regulation (EC) No 761/2009 and the US EPA Draft Ecological Effects Test Guideline OPPTS 850.5400.
Methods:
Following a preliminary range-finding test, Pseudokirchneriella subcapitata was exposed to an aqueous solution of the test item at concentrations of 10, 32, 100, 320 and 1000 mg active ingredient (ai)/L (three replicate flasks per concentration) for 96 hours, under constant illumination and shaking at a temperature of 24 ± 1 °C.
Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group, using a Coulter®Multisizer Particle Counter.
Results:
Exposure of Pseudokirchneriella subcapitata to the test item gave the following results:
Time Point (Hours) |
Response Variable |
EC50(mg ai/L) |
95% Confidence Limits (mg ai/L) |
No Observed Effect Concentration (NOEC) (mg ai/L) |
Lowest Observed Effect Concentration (LOEC) (mg ai/L) |
||
72 |
Growth Rate |
510 |
|
* |
|
32 |
100 |
Yield |
150 |
120 |
- |
190 |
32 |
100 |
|
Biomass |
160 |
140 |
- |
190 |
32 |
100 |
|
96 |
Growth Rate |
790 |
|
* |
|
100 |
320 |
Yield |
220 |
180 |
- |
260 |
10 |
32 |
|
Biomass |
180 |
140 |
- |
210 |
32 |
100 |
|
Analysis of the test preparations at 0 and 96 hours showed measured test concentrations to range from 93% to 105% of nominal and therefore the results are based on nominal test concentrations only, as this coincides within the acceptable range of between 80 and 120%.
A re-growth test was performed which showed the test item to be algistatic in effect.
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