Registration Dossier
Registration Dossier
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EC number: 250-480-2 | CAS number: 31138-65-5
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The study was conducted according to GLP and according to OECD guideline 203. The test item was exposed to Oncorhynchus mykiss for 96 hours under semi-static test conditions. The EC50 value after 96 hours was 1000 mg ai/L and the NOEC was 1000 mg/ai/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1 000 mg/L
Additional information
Introduction
A study was performed to assess the acute toxicity of the test item to rainbow trout (Oncorhynchus mykiss). The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No 203, "Fish, Acute Toxicity Test" referenced as Method C.1 of Commission Regulation (EC) No. 440/2008 and US EPA Draft Ecological Effects Test Guidelines OPPTS 850.1075 "Fish Acute Toxicity Test, Freshwater and Marine".
Methods
Following preliminary range-finding tests, fish were exposed, in groups of ten, to an aqueous solution of the test item over a range of concentrations of 100,180,320,560, and 1000 mg active ingredient (ai)/L for a period of 96 hours at a temperature of 13 °C to 14 °C under semi-static conditions. The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the test until termination after 96 hours.
Results
The 96-Hour LC50based on nominal test concentrations was greater than 1000 mg ai/L. The No Observed Effect Concentration was 1000 mg ai/L.
Analysis of the 1000 mg ai/L test preparation at 0, 24, 48, 72 and 96 hours showed measured test concentrations to range from 91% to 114% of nominal and so the results are based on nominal test concentrations only. The fresh test preparation at 48 hours was measured to be 184% of nominal, however, as the corresponding old preparation at 72 hours was near nominal it is considered that this result was anomalous and has no impact on the outcome of the test.
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