Fumes, silica

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

19 Nov. 1991 - 23 Nov. 1991

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Automatically generated during migration to IUCLID 6, no data available

IUPAC Name:

Automatically generated during migration to IUCLID 6, no data available

Details on test material:

Aerosil 200: approx. 99.8 % (SiO2): CAS-Name: Silica, amorphous,
fumed, cryst.-free; CAS-No.: 112945-52-5

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stirring for 20 h, then allowed to stand for 4 h before testing;
- Eluate: no, suspensions were tested
- Differential loading: yes, 1000 + 10,000 mg/L
- Controls: Synthetic medium from groundwater
- Evidence of undissolved material (e.g. precipitate, surface film, etc): The resulting suspensions at the beginning of the test were homogeneous and milky, at the end of the test in addition a layer of white, starchy flocks on the bottom of the vessels was observed.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Strain:
- Source: M.B. Ruysbroek B.V./Noordvliet 159, Maassluis
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 2.2 +-0.2 cm
- Weight at study initiation (mean and range, SD): 0.09 +-0.02 g
- Method of breeding:
- Feeding during test: no


ACCLIMATION
- Acclimation period: no data
- Type and amount of food: no data
- Feeding frequency: no data

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

none

Test conditions

Hardness:

204 mg/L as CaCO3

Test temperature:

24.5 - 25.3 °C

pH:

7.3 - 8.2

Dissolved oxygen:

7.1 - 8.2 mg/L

Salinity:

not applicable

Nominal and measured concentrations:

1000 and 10,000 mg SiO2/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass beaker, 2 L, 1000 ml test suspension
- Aeration: slight aeration (no data)
- Type of flow-through (e.g. peristaltic or proportional diluter): --
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): --
- Biomass loading rate: approx. 0.9 g fish/L


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: prepared from groundwater by addition of several salts
- Total organic carbon: 1.7 mg/L
- Alkalinity: -- no data
- Ca/Mg ratio: 1.83
- Conductivity: --



OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark
- Light intensity: -- no data


EFFECT PARAMETERS MEASURED (with observation intervals if applicable): survival. swimming behaviour, colour, respiratory function,
morphological and physiological changes that were visually observable at 24, 48, 72, and 96 h


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Justification for using less concentrations than requested by guideline: limit test, expected low toxicity
- Range finding study
- Test concentrations: --
- Results used to determine the conditions for the definitive study: --

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LL0

Effect conc.:

10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks:

0/20 animals died

Details on results:

- Behavioural abnormalities: none
- Observations on body length and weight: none
- Other biological observations: no particular findings
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: none

Results with reference substance (positive control):

not appliicable

Reported statistics and error estimates:

not appliicable

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Aerosil 200 was not acutely toxic to Brachydanio rerio at a loading of 10,000 mg/l.

Executive summary:

After 96 h of exposure all animals were alive and their conditiona (swimming behaviour, colour, respiratory function or any other visually observable morphological or behavioural criterion) was equal to that of the control animals.