Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1988
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1988

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
purity: 99.7 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebrabärbling
- Strain: Hamilton-Buchanan
- Source: West Aquarium, 3422 Bad Lauterberg
- Length at study initiation: 2.5 - 3.5 cm average 3.0 cm s= 0.28 cm
- Feeding during test: no
ACCLIMATION
- Acclimation period: about 14 days
- Acclimation conditions: same as test
- Type and amount of food: Tetra Min, Tetra Werke, Melle
- Feeding frequency: twice a day ad libitum before

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
no

Test conditions

Test temperature:
21 - 23 °C
pH:
5.7 - 8.1
Dissolved oxygen:
7.4 - 8.8 mg/L
Nominal and measured concentrations:
Nominal concentrations: 0, 180, 250, 350, 500 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass container (30cm x 22cm x 24cm)
- Type (delete if not applicable): open / closed
- Fill volume: 10 liters
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: composition according to ISO/DIS 7346/1


OTHER TEST CONDITIONS
- Photoperiod: 12 hours
- Light intensity: 700 Lux

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
180 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
250 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
350 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
other: LC90
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 350 - < 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: projecting opercula, sometimes increased respiration rate, sometimes hypoactivity, sometimes increased fright reaction.
In the group with 180 mg/L the fish showed no more symptoms after 48 hours.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The LC0 is 350 mg/L and LC50 between 350 and 500 mg/L. Therefore the substance is not subject for classification concerning the toxicity to fish.
Executive summary:

The substance was tested for toxicity to fish (Zebrabärbling) according to OECD guideline 203. The test design was devided in three groups: a: 0 and 180 mgL, b: 0 and 500 mg/L, c: 0, 250 and 350 mg/L. For each test 10 fish were used. After 96 hours only the test group with 500 mg/L showed letality (9/10) and symptoms. The groups with 180, 250 and 350 mg/L showed symptoms but no letality.

The substance is only poorly soluable in water, therefore residual undissolved material could be observed at the ground of the test container.