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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study conducted in compliance with GLP regulations

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guideline
according to guideline
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
(2-hydroxyethyl)(3-hydroxypropyl)dimethylammonium chloride
EC Number:
EC Name:
(2-hydroxyethyl)(3-hydroxypropyl)dimethylammonium chloride
Cas Number:
Molecular formula:
3-hydroxy-N-(2-hydroxyethyl)-N,N-dimethylpropan-1-aminium chloride
Details on test material:
- Name of substance (as cited in study report): DMAC TROCKEN FLUESSIG
- Chemical name: 1-Propanaminium, 3-hydroxy-N-(2-hydroxyethyl)-N, N-dimethyl-, chloride
- Molecular formular: C7 H18 N O2 Cl
- Molecular weight (g/mol): 183.7
- Aggregated state: solid
- Density (g/ml): 1.12
- Water solubility: miscible
- Colour: light brown
- Purity of test substance: 98.9 %
- Homogeneity: yes
- Instability against: temperature > 100 °C

Study design

Oxygen conditions:
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Inoculum: Municipal activated sludge from laboratory wastewater treatment plants fed with municipal sewage.
- Concentration of dry substance 30 mg/L
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance
Reference substance:

Results and discussion

% Degradation
% degradation (O2 consumption)
70 - 80
Sampling time:
28 d
Details on results:
- Duration of adaption phase (d): 10d
- Duration of degradation phase (d): 7
- Degradation of test substance at end of 10-d-window (% BOD/ThOD): 80 - 90 (Nitrification not considered)
- Degradation degree of test substance after 28 d (% BOD/ThOD): 70 - 80
- Degradation degree of test substance in abiotic control at end of test (% BOD/ThOD): 0 - 10

- Kinetic of test substance mean (in %):
= 0 after day 1
= 6 after day 11
= 18 after day 12
= 52 after day 13
= 60 after day 14
= 64 after day 15
= 86 after day 28

BOD5 / COD results

Results with reference substance:
- Degradation degree of reference substance after 14 days (% BOD/ThOD): 80 - 90
- Degradation degree in inhibition control after 14 days (% BOD/ThOD): 70 - 80

Applicant's summary and conclusion

Interpretation of results:
readily biodegradable