Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
November - March 1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1998
Report date:
1999

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Qualifier:
according to guideline
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
Qualifier:
according to guideline
Guideline:
other: EEC Directive 88/302/EEC (Activated Sludge Respiration Inhibition Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
(2-hydroxyethyl)(3-hydroxypropyl)dimethylammonium chloride
EC Number:
278-860-3
EC Name:
(2-hydroxyethyl)(3-hydroxypropyl)dimethylammonium chloride
Cas Number:
78182-00-0
Molecular formula:
C7H18NO2.Cl
IUPAC Name:
3-hydroxy-N-(2-hydroxyethyl)-N,N-dimethylpropan-1-aminium chloride
Details on test material:
- Name of test material (as cited in study report): DMAC Trocken Fluessig
- Chemical name: 1-Propanaminium, 3-hydroxy-N-(2-hydroxyethyl)-N, N-dimethyl-, chloride
- Molecular formular: C7 H18 NO2 Cl
- Batch no.: P 55
- Product no.: 097852
- Purity: no data (see Analytical Report ZAX No.: 98L00661)
- Molecular weight (g/mol): 183.7
- Aggregated state: solid
- Colour: light brown
- Water-solubility: miscible
- Instability against: heat > 100 °C
- Homogeneity: yes
- Remarks for storage: store at room temperature

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Inoculum: activated sludge from laboratory wastewater plants treating municipal and synthetic sewage
- Preparation of inoculum: inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated for 24h. 50 ml were added to test
volume of 250 ml to obtain a concentration of 1 g/L dry substance in the test
- The concentration of dry substance was 1 g/l

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
30 min

Test conditions

Test temperature:
20 +/- 2 °C
pH:
TEST SUBSTANCE
- pH-value before adding inoculum (after correction): 7.4
- pH-value after incubation time of 30 min: 7.5
REFERENCE SUBSTANCE
- pH-value before adding inoculum (after correction): 7.1 - 7.2
Dissolved oxygen:
TEST SUBSTANCE
- concentration at start (mg O2/L): 7.4
- concentration at end (mg O2/L): 5.3
REFERENCE SUBSTANCE:
- oxygen concentration at start (mg O2/L): 7.5 - 8.7
- oxygen concentration at end (mg O2/L): 5.5 - 8.4
Nominal and measured concentrations:
- test concentration (mg/L): 1000 (nominal)
Details on test conditions:
- test vessel: Erlenmeyer-vessel (nominal volume 250 ml)
- test volume: 250 ml
- synthetic medium: 8 ml/vessel 100-fold concentrated OECD Medium
- oxygen concentration during aeration: > 2.5 mg/L
- oxygen concentration immediately before measurement: > 6.5 mg/L
- duration of the measurement of oxygen consumption: 6 - 10 min
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
30 min
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
EC-VALUES:
duration: 30 min EC20: ca. 2.2 mg/L nominal test mat. respiration rate
duration: 30 min EC50: ca. 6.8 mg/L nominal test mat. respiration rate
duration: 30 min EC80: ca. 62 mg/L nominal test mat. respiration rate

highest concentration tested: 100 mg/L (nominal)



Applicant's summary and conclusion