2,4-dichlorobenzoyl chloride

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1981

Materials and methods

Test guideline

Qualifier:

no guideline available

Guideline:

other: Ames test but no guideline named

GLP compliance:

yes (incl. QA statement)

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Benzoylchloride, 2,4-dichloro-

IUPAC Name:

Benzoylchloride, 2,4-dichloro-

Constituent 2

Reference substance name:

2,4-dichlorobenzoyl chloride

EC Number:

201-936-4

EC Name:

2,4-dichlorobenzoyl chloride

Cas Number:

89-75-8

Molecular formula:

C7H3Cl3O

IUPAC Name:

2,4-dichlorobenzoyl chloride

Test material form:

other: clear liquid

Details on test material:

2,4-Dichlorobenzoyl chloride: purity: at least 99 %, Storage conditions: dark 22°C
At the day of the experiment the substance was dissolved in DMSO at the appropriate concentration.

Method

Target gene:

In the Salmonella typhimurium strains (TA 1535, TA 100, TA 1537, TA 1538, TA 98) the amino acid histidine locus is the target gene.
In the Escherichia coli strain (WP2 uvrA) the amino acid tryptophan locus is the target gene.

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2

Remarks:

additionan S. typhimurium TA 1538

Metabolic activation:

with and without

Metabolic activation system:

rat liver homogenate fraction( " S-9")

Test concentrations with justification for top dose:

The dose range of 6 different doses from 4 µg/plate to 10 000 µg/plate and from 0.8 µg/plate to 25000 µg/plate was used. Find attached the exact concentrations plus result in the attached background material.

Vehicle / solvent:

DMSO

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

Positive controls:

yes

Positive control substance:

9-aminoacridine

2-nitrofluorene

N-ethyl-N-nitro-N-nitrosoguanidine

benzo(a)pyrene

other: 2-Aminoanthracene

Details on test system and experimental conditions:

Mutagenicity Test: Top agar is prepared for Salmonella strain . Bacteria are grown overnight in nutrient broth.The suitable amount of bacteria in cell suspension is checked by nephelmetry. For inoculation, stock cultures which are stored at -80 °C are used.

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium, other: TA 100, TA 1535, TA 1537, TA 1538, TA98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Test results 2

Species / strain:

E. coli WP2 uvr A

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Additional information on results:

Sterility of S-9 Mix and the test compound was indicated by the absence of contamination on the test material and S-9 Mix sterility check plates. Control plates (background control and positive controls) gave expected number of colonies.

Applicant's summary and conclusion

Conclusions:

Summarizing, it can be stated that 2,4-Dichlorbenzoyl chloride is not mutagenic in these bacterial test system either with or without exogenous metabolic activiation at the dose level investigated.

Executive summary:

2,4-Dichlorobenzoyl chloride was tested for mutagenicity with strains TA 100, TA 1535, TA 1537, TA 1538, TA 98 of Salmonella typhimurium and Escherichia coli WP2uvrA.

Mutagenicity: In the absence of metabolic activation system the test compound did not show a dose dependent increase in number of revertants in any of the bacterial strains. Also in the presence of a metabolic activivation system, treatment of the cells with 2,4 -Dichlorobenzoyl chloride did not result in relevant increases in number of revertant colonies. It can be concluded that the substance is not mutagenic.