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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
skin sensitisation: in vivo (non-LLNA)
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
significant methodological deficiencies
Remarks:
The determination of dose is unclear, dead animals were removed from evaluation Redness and edema have been reported but assessment is nevertheless not sensitizing.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1972
Report date:
1972

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
no
Type of study:
other: modified Landsteiner

Test material

Constituent 1
Chemical structure
Reference substance name:
2,4-dichlorobenzoyl chloride
EC Number:
201-936-4
EC Name:
2,4-dichlorobenzoyl chloride
Cas Number:
89-75-8
Molecular formula:
C7H3Cl3O
IUPAC Name:
2,4-dichlorobenzoyl chloride
Constituent 2
Reference substance name:
Benzoylchloride, 2,4-dichloro-
IUPAC Name:
Benzoylchloride, 2,4-dichloro-
Test material form:
solid: crystalline
Details on test material:
light yellow, unpleasant ordor,cristalline powder

In vivo test system

Test animals

Species:
guinea pig

Study design: in vivo (non-LLNA)

Inductionopen allclose all
Vehicle:
other: Sesame oil 0.1 %
Concentration / amount:
Sensitizing injections:
0.1 % solution in sesame oil
First dose 0.05 mL, succeding dose 0.1 mL per animal

Challengeopen allclose all
Vehicle:
other: Sesame oil 0.1 %
Concentration / amount:
Sensitizing injections:
0.1 % solution in sesame oil
First dose 0.05 mL, succeding dose 0.1 mL per animal

No. of animals per dose:
test concentrations: 8
vehicle control and positive control: 4
Details on study design:
MAIN STUDY
A. INDUCTION EXPOSURE
- No. of exposures: three times per week
- Exposure period: three weeks
- Site: into the clipped back and flanks

B. CHALLENGE EXPOSURE
- No. of exposures: one time
- Day(s) of challenge: 10 days after last sensitizing dose.
Challenge controls:
Following the last sensitising dose, the animals were set aside for 10 days after a challenge dose (0.05 ml of freshly prepared solution) was administered.
There were no responses to the challenge dose.
Positive control substance(s):
yes
Remarks:
DNCB

Results and discussion

In vivo (non-LLNA)

Results
Reading:
other: After challenge
Hours after challenge:
48
Group:
test chemical
No. with + reactions:
8
Total no. in group:
8
Clinical observations:
no clinical observations
Remarks on result:
other: Reading: other: After challenge. . Hours after challenge: 48.0. Group: test group. No with. + reactions: 8.0. Total no. in groups: 8.0. Clinical observations: no clinical observations.

Any other information on results incl. tables

One animal showed slight swelling without erythema following one of the sensitizing doses which might be due to mechanical factores and do not represent true sensitizing response.

Applicant's summary and conclusion

Conclusions:
In conclusion it can be said that the test item is not sensitising to Guinea Pigs under the conditions of the test.