4,4'-methylenedi-2,6-xylenol

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Experimental data of various test chemical

Justification for type of information:

Experimental data of various test chemical from collection of data

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: As mentioned below

Principles of method if other than guideline:

WoE report is based on two toxicity study of microorganisms for the test chemical.

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

yes

Remarks:

Study 1: DMSO

Details on test solutions:

Study1: Stock solutions were prepared in dimethylsulfoxide (DMSO) at concentrations of 5, 10, 25, or 50 g/L.

Test organisms (species):

other: study1: Tetrahymena pyriformis study 2: Microregma sp.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

2 d

Remarks on exposure duration:

Study 2: 28 h

Test temperature:

study 1: 27±1°C

pH:

study 1: 7.35

Nominal and measured concentrations:

study 1: Stock solutions were prepared in dimethylsulfoxide (DMSO) at concentrations of 5, 10, 25, or 50 g/L.

Details on test conditions:

study 1:
Test vessel: 250-mL Erlenmeyer flasks containing 50 mL medium.
Each flask was inoculated with log-phase culture to attain a starting concentration of ~2,500 cells/ml.
Replicates: 3 or more

Reference substance (positive control):

not specified

Key result

Duration:

2 d

Dose descriptor:

other: IGC50

Effect conc.:

116.16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: 95% CI: 96-68-150-87

Duration:

28 h

Dose descriptor:

other: not specified

Effect conc.:

70 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Feeding behaviour

Remarks on result:

other: other details not known

Validity criteria fulfilled:

not specified

Conclusions:

The effect of the test chemical to aquatic micro organisms was in a range of 70- 116.16 mg/L.

Executive summary:

Data available from various sources for target chemical has been reviewed to determine the toxicity of the test chemical on microorganisms. The studies are as mentioned below:

Data from the peer reviewed journal outlines that, The microbial growth was evaluated using the tetrahymena pyriformis population growth assay. The test was conducted in the static conditions for the period of 48 hours. Based on the outcomes the median inhibitory growth concentration was determied to be 116.16 mg/L.

Another study from the secondary source, summarizes the effect of test chemical on the microregma sp. a ciliated protozoan. Based on the feeding behaviour the effective concentration was determied to be 70 mg/L.

The effect of the test chemical to aquatic micro organisms was in a range of 70- 116.16 mg/L.

Description of key information

The microbial growth was evaluated using the tetrahymena pyriformis population growth assay. The test was conducted in the static conditions for the period of 48 hours. Based on the outcomes the median inhibitory growth concentration was determied to be 116.16 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:

116.16 mg/L

Additional information

Data available from various sources for test chemical and read across chemical selected on the basis of structual similarity has been reviewed to determine the toxicity of the test chemical on microorganisms. The studies are as mentioned below:

Data from the peer reviewed journal outlines that, The microbial growth was evaluated using the tetrahymena pyriformis population growth assay. The test was conducted in the static conditions for the period of 48 hours. Based on the outcomes the median inhibitory growth concentration was determied to be 116.16 mg/L.

Another study from the secondary source, summarizes the effect of test chemical on the microregma sp. a ciliated protozoan. Based on the feeding behaviour the effective concentration was determied to be 70 mg/L.

The effect of the test chemical to aquatic micro organisms was in a range of 70- 116.16 mg/L.