4,4'-methylenedi-2,6-xylenol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

data from handbook or collection of data for various chemicals

Justification for type of information:

data from handbook or collection of data

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 236 (Fish embryo acute toxicity (FET) test)

Principles of method if other than guideline:

The effect of test chemical was studied on the early life stage of zebrafish embryos.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

yes

Remarks:

study 1: DMSO

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Common name: Zebrafish

Test type:

other: Study 1: semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Study 1: The embryos were observed for the 24 h more after total exposure duration of 96 h.

Nominal and measured concentrations:

Study 1: Nominal concentrations: 0, 0.5, 1, 2, 5, 10, 20 and 50 μM,

Details on test conditions:

Study 1:
Number of species: 20 embryos
Number of replicates 3 replicates

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.77 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 1st study

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 2nd study

Validity criteria fulfilled:

not specified

Conclusions:

The range of median lethal concentration of the test chemical was determined to be 1.77- 5.0 mg/L.

Executive summary:

Data available from various sources for test chemical has been reviewed to determine the toxicity of the test chemical on fish: the studies are as mentioned below.

The Primary study outlines the effect of test chemical on the early life stage of zebrafish the test was conducted in accordance with OECD 236 guidelines, fish embryo toxicity test. Seven different concentrations was selected to evaluate the median lethal concentration i.e., 0.5, 1, 2, 5, 10, 20 and 50 μM. Test was conducted for the period of 120 h, in semi static conditions, and the median lethal concentration for 96 h was determined to be 1.77 mg/L (5.2µM).

The Secondary study outlines the effect of test chemical on the fish for the period of 96.0 h. the test was conducted in accordance with OECD 203 guidelines. After the exposure period the median lethal concentration was determiend to be >5 mg/L.

The toxicity range of the test chemical was determined to be 1.77 -5.0 mg/L. As per new CLP classification criteria the test chemical can be classified into aquatic chronic category 2.

Description of key information

The range of median lethal concentration of the test chemical was determined to be 1.77- 5.0  mg/L..

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1.77 mg/L

Additional information

Data available from various sources for test chemical and read across chemical has been reviewed to determine the toxicity of the test chemical on fish: the studies are as mentioned below.

The Primary study outlines the effect of test chemical on the early life stage of zebrafish. the test was conducted in accordance with OECD 236 guidelines, fish embryo toxicity test. Seven different concentrations was selected to evaluate the median lethal concentration i.e., 0.5, 1, 2, 5, 10, 20 and 50 μM. Test was conducted for the period of 120 h, in semi static conditions, and the median lethal concentration for 96 h was determined to be 1.77 mg/L (5.2µM).

The Secondary study outlines the effect of test chemical on the fish for the period of 96.0 h. the test was conducted in accordance with OECD 203 guidelines. After the exposure period the median lethal concentration was determiend to be >5 mg/L.

The toxicity range of the test chemical was determined to be 1.77 -5.0 mg/L. As per new CLP classification criteria the test chemical can be classified into aquatic chronic category 2.