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EC number: 203-904-5
CAS number: 111-75-1
Desmodesmus subspicatus_OECD 201: ErC50
(72h): 30 mg/L (extrapolated), ErC10 (72h): 2.4 mg/L, EbC50 (72h): 9.9
mg/L, EyC50 (72h): 5.2 mg/L, NOEC (72h): 0.39 mg/L
ECOSAR v1.11_green algae_Chronic Value (ChV): 70.44 mg/L (baseline
toxicity) and 5.591 mg/L (SAR "Aliphatic Amines").
The key study to evaluate the toxicity to
aquatic algae and cyanobacteria of the substance was experimentally
performed by Muckle (2013) according to OECD 201 and EU Method C.3. In
addition, supporting information based on SAR (structure - activity
relationship) estimations by ECOSAR v1.11 (EPIWIN software) is available
(Chemservice S.A., 2018).
The acute toxicity to aquatic algae of
n-Butylaminoethanol was tested according to OECD Guideline 201 and EU
Method C.3 in a static freshwater test with Desmodesmus subspicatus (former
name: Scenedesmus subspicatus) as test organism (Muckle, 2013).
This key study is in compliance with GLP and was performed using
five concentrations ranging from 0.1 to 10 mg/L nominal concentration.
Incubation time was 72h. The cell concentration of each replicate was
determined by measuring the absorption of the solutions at 440 nm every
24h with a spectral photometer. The cell density of the cultures was
calculated based on the correlation curve between the adsorption and the
cell density of the cultures determined by microscope counts. Growth
rate µ, area under the growth curve (AUC) and the yield were determined
from the cell densities at the respective observation times.
Significant inhibition of algal growth
was observed at the nominal concentrations 1.0 mg/L, 3.2 mg/L and 10
mg/L. At each sampling date, the content of the test item in the test
solutions was determined using HPLC-MS. The recovery after 72h was in a
range between 103 % and 110 % of the measured start concentration.
Therefore the test item can be stated as stable under test conditions.
The measured concentrations were in a range between 106 % and 143 % of
the nominal concentrations. Therefore the biological results were based
on the geometric mean of the measured concentrations. The EC50 values of
potassium dichromate (positive control) were tested in a separate
reference test (GLP study no. 201206R301). The values lay within the
normal range of the laboratory.
Based on the growth rate (72h), a NOErC of
0.39 mg/L, a LOErC of 1.2 mg/L, an ErC10 of 2.4 mg/L and an ErC50 of 30
mg/L (extrapolated) are reported. With regard to the AUC (72h, performed
according to EU Method C.3), a NOEbC of 0.39 mg/L, a LOEbC of 1.2 mg/L
and an EbC50 of 9.9 mg/L are determined. Based on the yield (72h), a
NOEyC of 0.39 mg/L, a LOEyC of 1.2 mg/L and an EyC50 of 5.2 mg/L are
The chronic toxicity to green algae of
Butylaminoethanol was further predicted by the computer program ECOSAR
v1.11 (EPIWIN software) by US-EPA (Chemservice S.A., 2018) as supporting
extensive set of structure – activity relationships (SARs) is utilized
by the computer program, to predict the acute and chronic toxicity for
three trophic levels for aquatic species, based on the neutral organics
SAR (Baseline Toxicity). The
mode of toxic action for most neutral organic chemicals is narcosis, and
many chemical classes present toxicity to organisms via narcosis (i.e.
ethers, alcohols, ketones). However, some organic chemical classes have
been identified as having a more specific mode of toxicity. These are
typically organics that are reactive and ionizable and that exhibit
excess toxicity in addition to narcosis (i.e. acrylates, epoxides,
anilines). Regarding long-term toxicity, the so-called Chronic Value
(ChV) is predicted, which is defined
as the geometric mean of the Lowest Observed Effect Concentration (LOEC)
and the No Observed Effect Concentration (NOEC). It can just classify a
substance according to a low, moderate or high toxicity concern. For the
test substance concerning long-term toxicity to green algae a ChV(4d) of
70.44 mg/L is predicted for baseline toxicity and 5.591 mg/L applying
the SAR "Aliphatic Amines", indicating a moderate toxicity concern. No
GLP criteria are applicable for the usage of this tool, but due to the
fact that it is a scientifically accepted calculation method the
estimations performed are reliable with restrictions and can be used for
the chemical safety assessment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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