2-butylaminoethanol

Endpoint summary

Additional information

Toxicity to fish

 

Leuciscus idus was used in an experiment which was conducted according to DIN 38412, part 15 (BASF AG, 1989). Since no mortality occurred up to the highest tested concentration of 100 mg/L, the LC50(96h) is considered to be > 100 mg/L. Thus, Butylaminoethanol can be considered as non-toxic to freshwater fish.

Based upon the chemical safety assessment and due to animal welfare, no long-term investigation is triggered. An allusion to long-term toxicity is given by the prediction using ECOSAR v1.00 (Chemservice S.A., 2011). The result revealed a Chronic Value (ChV, 30d) far above 10 mg/L, which is the trigger value for a low toxicity concern. Taking into consideration results from short-term toxicity tests on fish, Daphnia and algae, there is a high probability that the most sensitive species (algae) has already been examined and that a further long-term result from fish would not be lower than the data already available.  

Moreover, the exposure levels estimated in all relevant scenarios do not exceed the appropriate PNEC (all risk characterization ratios are under 1.0), and the likelihood and severity of an event occurring due to the physicochemical properties of the substance in the aquatic environment are negligible..

Toxicity to aquatic invertebrates

 

Short-term toxicity towardsDaphnia magnawas investigated according to OECD Guideline 202 / EU Method C.2 (Muckle, 2013). Five nominal concentrations were used as followed: 0 (control), 10, 22, 46, 100 and 220 mg/L. Measured concentrations at the end were in a range between 107 % and 122 % of the nominal concentration and in a range of 104 - 123 % of the measured start concentration, respectively. The following results were reported: EC50(48h): 180 mg/L and a NOEC(48h) of 52 mg/L. All validity criteria were met with exception of a pH deviation, since the pH at the beginning in the higher concentrated treatments was untypically high. Thus might be caused by the test substance properties, however, according to the applied guidelines, no pH adjustment was performed.Toxic effects may be caused at least partly by a pH-shift in the test solutions at concentrations above 46 mg/L.

Based on the chemical safety assessment (CSA), no long-term toxicity investigations are triggered in accordance to REACH, Annex IX, Section 9.1.5, column 2.The exposure levels estimated in all relevant scenarios do not exceed the appropriate PNEC, and the likelihood and severity of an event occurring due to the physicochemical properties of the substance in the aquatic environment are negligible.

Toxicity to aquatic algae

 

The acute toxicity to aquatic algae was tested according to OECD Guideline 201 and EU Method C.3 withDesmodesmus subspicatusas test organism (Muckle, 2013). Five test concentrations were used, ranging from 0.1 to 10 mg/L (nominal). Significant inhibition of algal growth was observed at the concentrations of 1.0 mg/L, 3.2 mg/L and 10 mg/L. The measured concentrations were in a range between 106 % and 143 % of the nominal concentrations. Therefore the biological results were based on the geometric mean of the measured concentrations. Based on the growth rate (72h), an ErC10 of 2.4 mg/L[#], a NOErC of 0.39 mg/L[#], a LOErC of 1.2 mg/L and an ErC50 of 30 mg/L (extrapolated) are reported. With regard to the AUC (72h, performed according to EU Method C.3), a NOEbC of 0.39 mg/L, a LOEbC of 1.2 mg/L and an EbC50 of 9.9 mg/L are determined. Based on the yield (72h), a NOEyC of 0.39 mg/L, a LOEyC of 1.2 mg/L and an EyC50 of 5.2 mg/L are reported. Additionally, for the test substance concerning long-term toxicity to green algae a ChV(4d) of 70.44 mg/L is predicted for baseline toxicity and a ChV(4d) of 5.991 mg/L is predicted applying the SAR 'Aliphatic Amines', indicating a moderate toxicity concern.

 

[#] The ErC10 instead of the NOErC was considered as the key value for the chemical safety assessment. According to the Guidance on information requirements and chemical safety assessment Chapter R.10: Characterisation of dose [concentration]-response for environment "an EC10 for a long-term test which is obtained using an appropriate statistical method (usually regression analysis) will be used preferentially. [...] There has been a recommendation within OECD in 1996 to phase out the use of the NOEC, in particular as it can correspond to large and potentially biologically important magnitudes of effect. The advantage of regression method for the estimation of ECx is that information from the whole concentration-effect relationship is taken into account and that confidence intervals can be calculated. These methods result in an ECx, where x is a low effect percentile (e.g. 5-20%). It makes results from different experiments more comparable than NOECs." Therefore, the EC10 instead of the NOEC has been used to derive the classification.

Toxicity to microorganisms

 

BASF SE (2013) investigated the toxicity towards aquatic microorganisms in an experiment conducted according to OECD Guideline 209 / EU Method C.11. The EC10(3h) is reported as 350 mg/L, EC50(3h) and EC80(3h) were > 1000 mg/L.