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EC number: 480-410-8 | CAS number: 13482-23-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 Feb - 17 Jul 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Internal standard solution:
30.0 mg Cyclododecane were dissolved in methanol and made up with methanol to the mark in a 20 ml volumetric flask to prepare a solution of 1500 µg/L. This solution is used as internal standard solution.
Standard solutions:
50.0 mg of the analytical standard were dissolved in acetonitrile and made up with acetonitrile to the mark in a 25 ml volumetric flask (2000 mg/L). 1 ml of this solution was diluted to 20 ml with acetonitrile to prepare a stock solution of 100 mg/L. Defined volumes of this stock solution and an aliquot of the internal standard solution were added to 15 ml water, 2 pellets potassium hydroxide and about 4.5 g sodium sulfate resulting in standard solutions in a range of 0.05 to 12.0 mg test item /I water.
These mixtures were extracted with 4 ml diethylether. The organic extract was used to calibrate the GC-system.
Although the overall method sensitivity may be better, the concentration of the lowest used calibration solution is employed as the limit of quantification.
Sample storage conditions before analysis:
Routinely, the samples are analysed immediately. Only in exceptional cases, they are stored overnight deeply frozen and protected from light. - Vehicle:
- no
- Details on test solutions:
- A direct weighing was prepared to give the desired test concentration. To achieve this 125.3 mg of the test item were added to 1 litre of dilution water and treated with an ultra turrax for 60 seconds at 8000 rpm and afterwards stirred for 1 hour on a magnetic stirrer.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Source: on-axenic strain of the test species obtained from The Collection of Algal Cultures' of the Institute of Plant Physiology at the University of Gottingen (Germany).
Maintenance of stock cultures: Exponentially-growing stock cultures are maintained in the test facility under constant temperature conditions(23 +/- 2°C) at a light intensity in the range 60 - 120jiE. x m"2 x s"1 (measured in the range 400 to 700 nm using a spherical quantum flux meter). The nutrient medium (according to BRINGMANN & KUHN (1977)is renewed once a week. Cell density measurements are made using a microcell counter. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Hardness:
- None reported.
- Test temperature:
- None reported.
- pH:
- 8.0-8.8
- Nominal and measured concentrations:
- nominal: 100 mg/L
meassured: 87.4 - 92.1 mg/L (0 h), 90.9 - 93.9 mg/L (72 h) - Details on test conditions:
- Test vessels: 300 ml Erlenmeyer flasks with cotton stoppers
Culturing apparatus: Light chamber in which a temperature in the range 21°C to 25°C can be maintained at +/- 2°C, and continuous uniform illumination is provided in the spectral range 400 to 700 nm.
Light intensity: At the average of the test solutions, a light intensity in the range 60 to 120 μE x m-2 x s-1, or an equivalent range of 4000 to 8000 1x, is recommended to use.
Cell densities are measured in a microcell counter or, alternatively, are determined by means of a microscopic counting chamber.
Experimental design: 1 test concentration plus 1 control; 3 replicates per concentration, 6 replicates per control; initial cell density in the test cultures approximately 5000 cells per millilitre; additionally highest test concentration without algae
Criteria of effects: The criteria of adverse effects used in this study were the item-induced inhibition of growth [b] and growth rate [r], respectively, of the algal population. - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- All results are expressed in terms of nominal concentrations. Recovery rates ranged from 87.4 - 92.1% of nominal values at 0 hours, and from 90.9 - 93.9% of nominal values at 72 hours, respectively.
- Results with reference substance (positive control):
- None.
- Validity criteria fulfilled:
- yes
- Remarks:
- See 'Any other information on results incl. tables'.
- Conclusions:
- An 72h EC50 of > 100 mg/L was determined for the test substance, as no toxic effects against algae were observed.
- Executive summary:
No toxic effects against algae at a concentration of 100 mg/l.
Reference
Validity criteria for the measurement of the algae toxicity
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
Exponentially growing test organisms are exposed to the test substance in batch cultures over a period of normally 72 hours. | Test organisms: Desmodesmus subspicatus Test period: 72 h | Yes |
The biomass in the control cultures should have increased exponentially by a factor of at least 16 within the 72-hour test period. | Cell density in the control flasks after 3 days showed a reproduction rate that was greater than a factor of 16 (factor 81). | Yes |
The mean coefficient of variation for section-by-section specific growth rates in the control cultures must not exceed 35%. | In this specific study, the observed value is 18.6%. | Yes |
The coefficient of variation of average specific growth rates during the whole test period in replicate control cultures must not exceed 10%. | It was 2.05% in this study. | Yes |
For the final definitive test at least five concentrations, arranged in a geometric series with a factor not exceeding 3.2, should be selected. For test substances showing a flat concentration response curve a higher factor may be justified. The concentration series should preferably cover the range causing 5-75 % inhibition of algal growth rate. | Test concentration no.: limit test (100 mg/L). | Not applicable |
The cultures should be maintained at a temperature in the range of 21 to 24°C, controlled at ± 2°C. | 21°C to 25°C can be maintained at +/- 2°C. | Yes (a small deviation of 1 °C is acceptable) |
The pH of the control medium should not increase by more than 1.5 units during the test. | pH in the control medium after 0 and 72 h was 8.1 - 8.6, respectively. | Yes |
Description of key information
An 72h EC50 of > 100 mg/L was determined for the test substance, as no toxic effects against algae were observed.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
Additional information
Should read > 100 mg/L.
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