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Ecotoxicological information

Toxicity to soil microorganisms

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Administrative data

Endpoint:
toxicity to soil microorganisms
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study predates implementation of GLP and/or development of study guidelines, published in peer-reviewed journal, adequate for assessment.

Data source

Reference
Reference Type:
publication
Title:
Effects of Trace Elements on Arylsulfatase Activity in Soils
Author:
Al-Khafaji AA, Tabatabai MA
Year:
1978
Bibliographic source:
Soil Science Vol 127, No 3 1979

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
The study evaluated the relative effects of equimolar concentrations of trace elements on arylsulfatase activity in soils.
GLP compliance:
no

Test material

Constituent 1
Reference substance name:
Silver nitrate
EC Number:
231-853-9
EC Name:
Silver nitrate
Cas Number:
7761-88-8
IUPAC Name:
silver(1+) nitrate
Details on test material:
Ag added as sulfate. Fisher certified reagent-grade.

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
no data

Test substrate

Details on preparation and application of test substrate:
1g soil in 50-ml Erlenmeyer flask, treated with 1 ml of solution, containing either 2.5 or 25 μmole of trace element.

Test organisms

Test organisms (inoculum):
soil

Study design

Total exposure duration:
24 h
Remarks:
Drying time. Arylsufatase activity assayed after 30-min equilibration from addition of solution to soil.

Test conditions

Test temperature:
Dried at 105 degrees C for 24 hr
Details on test conditions:
Surface soils - 0-15 cm. Soils air dried, crushed to pass 2mm screen. 30 Min equilibration then arylsulfatase activity assayed. Results compared to control, duplicate determinations calculated on an oven-dry basis.
Nominal and measured concentrations:
2.5 or 25 μmole trace element.
Reference substance (positive control):
yes
Remarks:
1g soil treated with 1ml water

Results and discussion

Effect concentrations
Duration:
24 h
Dose descriptor:
other: inhibition of arylsulfatase activity
Effect conc.:
96 other: %
Details on results:
Authors state average 96% inhibition of arylsulfatase activity in soil specified

Applicant's summary and conclusion

Conclusions:
Authors state average 96% inhibition of arylsulfatase activity in soil specified.
Executive summary:

This study predates the implementation of GLP and/or study guidelines. It was published in a peer-reviewed journal, and is considered adequate for use as a supporting study for this endpoint.

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