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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets generally accepted scientific standards, well documented and acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Range-Finding Toxicity Data: List IV
Author:
Smyth HF, Carpenter CP, Weil CS
Year:
1951
Bibliographic source:
Arch.Ind.Hyg.Occup.Med. 4: 119-122

Materials and methods

Principles of method if other than guideline:
Primary skin irritation on rabbits is recorded in a 10. grade ordinal series and is based upon the severest reaction that develops on the clipped skin of each of five albino rabbits within 24 hours of the uncovered application of 0.01 ml of undiluted sample or of solutions in water, propylene glycol, or acetone, scored somewhat similarly to the method reported by Draize and associates. Grade 1 shows no reaction whatever from undiluted sample, 2 an average reaction equivalent to a trace of capillary injection, 3 strong capillary injection, 4 slight erythema, 5 strong erythema, edema or slight necrosis, 6 and higher necrosis, where undiluted and Grade 10 indicates necrosis from a 0.01% solution. In Grade 6 a 10 per cent solution gives no reaction more severe than edema, 7 a 1 per cent solution, 8 a 0.1 per cent solution, 9 a 0.01 per cent solution, and 10 any weaker solution.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Isoquinoline
EC Number:
204-341-8
EC Name:
Isoquinoline
Cas Number:
119-65-3
Molecular formula:
C9H7N
IUPAC Name:
isoquinoline
Details on test material:
- Name of test material (as cited in study report): Iso quinoline
no further data

Test animals

Species:
rabbit
Strain:
other: albino rabbit
Details on test animals or test system and environmental conditions:
no data

Test system

Type of coverage:
open
Preparation of test site:
shaved
Vehicle:
unchanged (no vehicle)
Controls:
not specified
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.01 ml
Duration of treatment / exposure:
24 hours
Observation period:
24 hours
Number of animals:
5

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 24 hours
Score:
5
Max. score:
10
Reversibility:
no data
Remarks on result:
other: Grade 5 is described as: strong erythema, edema or slight necrosis after open application of 0.01 ml for 24 hours

Applicant's summary and conclusion

Interpretation of results:
irritating
Remarks:
Migrated information Criteria used for interpretation of results: EU

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