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EC number: 204-341-8 | CAS number: 119-65-3
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific standards, well documented and acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenicities of quinoline and its derivatives
- Author:
- Nagao M, Yahagi T, Seino Y, Sugimura T, Ito N
- Year:
- 1 977
- Bibliographic source:
- Mutation Research 42: 335-342
Materials and methods
- Principles of method if other than guideline:
- The mutagenicity of isoquinoline was tested in Salmonella typhimurium tester trains TA98 and TA100.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Isoquinoline
- EC Number:
- 204-341-8
- EC Name:
- Isoquinoline
- Cas Number:
- 119-65-3
- Molecular formula:
- C9H7N
- IUPAC Name:
- isoquinoline
- Details on test material:
- - Name of test material (as cited in study report): isoquinoline
- Analytical purity: purest grade available
Constituent 1
Method
- Target gene:
- his gene
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 98
- Additional strain / cell type characteristics:
- not applicable
- Species / strain / cell type:
- S. typhimurium TA 100
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 mix
- Test concentrations with justification for top dose:
- 1-20 umol/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: [no data]
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Positive control substance:
- not specified
- Details on test system and experimental conditions:
- Assay was carried out as described by Ames et al. with some modifications. Chemicals freshly dissolved in 100 ul of DMSO were pre-incubated at 37°C for 20 min with 0.5 ml of S-9 mix or 0.5 ml of 0.1 M sodium phosphate buffer (pH 7.4) and 0.1 ml of bacterial culture. Two ml of molten soft agar at 45°C were added, and the resulting mixture was poured over 25 ml of minimal-glucose agar containing 0.1 umol of L-histidine and 0.1 umol of biotin. After 2-day incubation, colonies of histidine prototroph were counted as revertants.
- Evaluation criteria:
- no data
- Statistics:
- no data
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 7 umol/plate and up (without S-9 mix), 10 umol/plate with S-9 mix
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 7 umol/plate and up (without S-9 mix), 10 umol/plate with S-9 mix
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
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