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EC number: 930-592-4 | CAS number: -
The aim of the study was to investigate acute toxic effects of the test substance - Reaction product of Distillates (petroleum), acid-treated heavy naphthenic and calcium oxide - after a single oral administration to Wistar rats.
The testing was performed according to the Method B.1 tris: Acute Oral Toxicity - Acute Toxic Class Method, Council Regulation (EC) No.440/2008, published in O.J. L 142, 2008.
According to the study results the value of LD50 of the test substance for female rats is higher than 2000 mg/kg of body weight.
Test substance was administered in a single dose as a solution in olive oil (vehicle), given orally via gavage to two groups of three female Wistar rats. The dosing was performed sequentially in two groups of three females: group No.1 - first step using the starting dose of 2000 mg/kg of body weight and group No.2 - second step using the same dose.
The test substance administered at the dose of 2000 mg/kg caused no death of animals. No clinical signs of intoxication were observed during study in all six animals. No pathologic macroscopic changes were diagnosed during pathological examination.
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