Silane, (isocyanatomethyl)dimethoxymethyl-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2002-08-19 to 2003-01-23

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Good laboratory practice guideline study (OECD).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

453-230-2

EC Name:

-

Cas Number:

406679-89-8

Molecular formula:

Hill formula: C5H11NO3Si CAS formula: C5H11NO3Si

IUPAC Name:

(isocyanatomethyl)dimethoxymethylsilane

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
not applicable

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: nominal 100 mg test item/L

- Sampling method: Duplicate samples from the freshly prepared test media of the only test concentration and the control were taken at the start of the test. Additionally samples were collected at the end of the test.

- Sample storage conditions before analysis: All samples were analysed directly after sampling

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION

- Method: Since the test item hydrolyses in water very quickly, in the present test the hydrolysis products of the test item were tested, according to the draft guidance document on aquatic toxicity testing of difficult substances. Therefore, the test item was mixed into test water at a concentration of 100 mg/L and was stirred for 24 hours. Thus, the main part of the test item at the start of the test were hydrolysis products.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna (Straus), clone 5
- Source: supplied by Umweltbundesamt, Institut für Wasser-, Boden-, und Lufthygiene, Berlin, Germany in 1997
- Age at study initiation (mean and range, SD): 6 - 22.5 hours old
- Method of breeding: similar water quality as in test (pH, main ions, total hardness)


ACCLIMATION
- Acclimation period: 6 hours
- Acclimation conditions (same as test or not):same as test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

not applicable

Test conditions

Hardness:

2.5 mmol/l as CaCO3

Test temperature:

21 °C

pH:

7.9 to 8.0

Dissolved oxygen:

at least 8.1 mg/l

Salinity:

freshwater, not applicable

Nominal and measured concentrations:

100 mg/l (nominal) 83 mg/l (mean, measured);

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass beakers of 250 ml filled with 150 ml.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates):2
- No. of vessels per control (replicates):2


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: 4:1


OTHER TEST CONDITIONS
- Adjustment of pH: buffered solution



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : EC50 at 24 and 48 h

Reference substance (positive control):

yes

Remarks:

For the evaluation of the quality of the Daphnia clone and the experimental conditions the substance potassium dichromate p.A. is tested at least twice a year to demonstrate satisfactory test conditions in the study lab.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Since it is possible to recalculate the values to the initially applied concentration of test item, the reported analytical results are related to test item. At the start of the test just before introduction of the Daphnia 88 % of nominal, recalculated to test item, were found. After 48 hours test duration 78 % of the nominal value were determined. Thus, during the test period of 48 hours the Daphnia were exposed to the hydrolysis products of a mean of 83 mg test item/L. Since in the
mean 83 % of nominal were found, the reported results are related to the nominal concentration of 100 mg test item/L.

Results with reference substance (positive control):

not given

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Substance is not toxic or harmful to daphnids.

Executive summary:

The substance was administered to Daphnia magna at a limit concentration of 100 mg/l (nominal) according to OECD guideline 202. No immobilization occured neither after 24 hours nor after 48 h.