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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2002-08-19 to 2003-01-23
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Good laboratory practice guideline study (OECD).

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report Date:
2003

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
not applicable

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: nominal 100 mg test item/L

- Sampling method: Duplicate samples from the freshly prepared test media of the only test concentration and the control were taken at the start of the test. Additionally samples were collected at the end of the test.

- Sample storage conditions before analysis: All samples were analysed directly after sampling

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION

- Method: Since the test item hydrolyses in water very quickly, in the present test the hydrolysis products of the test item were tested, according to the draft guidance document on aquatic toxicity testing of difficult substances. Therefore, the test item was mixed into test water at a concentration of 100 mg/L and was stirred for 24 hours. Thus, the main part of the test item at the start of the test were hydrolysis products.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna (Straus), clone 5
- Source: supplied by Umweltbundesamt, Institut für Wasser-, Boden-, und Lufthygiene, Berlin, Germany in 1997
- Age at study initiation (mean and range, SD): 6 - 22.5 hours old
- Method of breeding: similar water quality as in test (pH, main ions, total hardness)


ACCLIMATION
- Acclimation period: 6 hours
- Acclimation conditions (same as test or not):same as test

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
not applicable

Test conditions

Hardness:
2.5 mmol/l as CaCO3
Test temperature:
21 °C
pH:
7.9 to 8.0
Dissolved oxygen:
at least 8.1 mg/l
Salinity:
freshwater, not applicable
Nominal and measured concentrations:
100 mg/l (nominal) 83 mg/l (mean, measured);
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass beakers of 250 ml filled with 150 ml.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates):2
- No. of vessels per control (replicates):2


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: 4:1


OTHER TEST CONDITIONS
- Adjustment of pH: buffered solution



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : EC50 at 24 and 48 h
Reference substance (positive control):
yes
Remarks:
For the evaluation of the quality of the Daphnia clone and the experimental conditions the substance potassium dichromate p.A. is tested at least twice a year to demonstrate satisfactory test conditions in the study lab.

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Since it is possible to recalculate the values to the initially applied concentration of test item, the reported analytical results are related to test item. At the start of the test just before introduction of the Daphnia 88 % of nominal, recalculated to test item, were found. After 48 hours test duration 78 % of the nominal value were determined. Thus, during the test period of 48 hours the Daphnia were exposed to the hydrolysis products of a mean of 83 mg test item/L. Since in the
mean 83 % of nominal were found, the reported results are related to the nominal concentration of 100 mg test item/L.
Results with reference substance (positive control):
not given

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Substance is not toxic or harmful to daphnids.
Executive summary:

The substance was administered to Daphnia magna at a limit concentration of 100 mg/l (nominal) according to OECD guideline 202. No immobilization occured neither after 24 hours nor after 48 h.