2,6-dimethylpyridine

Endpoint summary

Description of key information

Skin irritation:

The test substance was found to be irritating to the rabbit skin.

Eye irritation:

The test substance was found to be irritating to the rabbit eye.

Key value for chemical safety assessment

Skin irritation / corrosion

Link to relevant study records

Reference

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Remarks:

Read across data

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from secondary literature

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

GLP compliance:

not specified

Specific details on test material used for the study:

Substance name: 2-Picoline
CAS no.: 109-06-8
Mol. wt.: 93.13
Smiles: CC1=CC=CC=N1
Physicl state: Liquid
Purity: Not specified

Species:

rabbit

Strain:

other: Albino

Details on test animals or test system and environmental conditions:

not specified

Type of coverage:

not specified

Preparation of test site:

not specified

Vehicle:

not specified

Controls:

not specified

Amount / concentration applied:

not specified

Duration of treatment / exposure:

not specified

Observation period:

4, 24 and 48 hours

Number of animals:

6

Details on study design:

not specified

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 4, 24 and 48 hours

Reversibility:

not specified

Remarks on result:

positive indication of irritation

Interpretation of results:

Category 2 (irritant) based on GHS criteria

Conclusions:

The test substance was found to be irritating to the rabbit skin.

Executive summary:

In vivo skin irritation study was performed in rabbits using test chemical 2 -Picoline (CAS no.: 109 -06 -8, E.C. no.: 203 -643 -7). 6 Albino rabbits were treated with the test chemical. Test chemical was administered dermally to the animals and were further observed for clinical signs of irritation at 4, 24 and 48 hours. Moderate erythema was observed. Hence, the test chemical can be considered as "Category 2" according to the CLP.

Endpoint conclusion

Endpoint conclusion:

adverse effect observed (irritating)

Eye irritation

Link to relevant study records

Reference

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Remarks:

Read across data

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from secondary literature

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

GLP compliance:

not specified

Species:

rabbit

Strain:

not specified

Details on test animals or tissues and environmental conditions:

not specified

Vehicle:

not specified

Controls:

not specified

Amount / concentration applied:

not specified

Duration of treatment / exposure:

not specified

Observation period (in vivo):

not specified

Number of animals or in vitro replicates:

not specified

Details on study design:

not specified

Irritation parameter:

other: conjunctival and corneal injury

Remarks on result:

positive indication of irritation

Interpretation of results:

Category 2 (irritating to eyes) based on GHS criteria

Conclusions:

The test substance was found to be irritating to the rabbit eye.

Executive summary:

In vivo eye irritation study was performed in rabbits using test chemical 2 -Picoline (CAS no.: 109 -06 -8, E.C. no.: 203 -643 -7). Rabbits were treated with the undiluted test chemical with and without washing. Animals were further observed for clinical signs of irritation. There was slight pain upon direct contact followed by severe conjunctival and corneal injury. The test substance was severely irritating to rabbit eye. Hence, the test chemical can be considered as "Category 2" according to the CLP.

Endpoint conclusion

Endpoint conclusion:

adverse effect observed (irritating)

Respiratory irritation

Endpoint conclusion

Endpoint conclusion:

no study available

Additional information

Skin irritation:

1. In vivo skin irritation study was performed in rabbits using testchemical 2 -Picoline (CAS no.: 109 -06 -8, E.C. no.: 203 -643 -7). 6 Albino rabbits were treated with the test chemical. Test chemical was administered dermally to the animals and were further observed for clinical signs of irritation at 4, 24 and 48 hours. Moderate erythema was observed. Hence, the test chemical can be considered as "Category 2" according to the CLP.

2. In vivo skin irritation study was performed in rabbits using testchemical 2 -Picoline (CAS no.: 109 -06 -8, E.C. no.: 203 -643 -7). Rabbits were treated with the test chemical. Test chemical was administered dermally to the animals onto the intact and abraded skin. Animals were further observed for clinical signs of irritation. Severe burning was accompanied by severe hyperemia and moderate edema after single application.Severe scar with moderate scabbing remained after 21 days. The test item was severely irritating to rabbit skin. Hence, the test chemical can be considered as "Category 2" according to the CLP.

3. In vivo skin irritation study was performed in rabbits using testchemical 3 -Picoline (CAS no.: 108 -99 -6, E.C. no.: 203 -636 -9). 6 Albino rabbits were treated with the test chemical. Test chemical was administered dermally to the animals and were further observed for clinical signs of irritation. Irritation was observed. Hence, the test chemical can be considered as "Category 2" according to the CLP.

Eye irritation:

1. In vivo eye irritation study was performed in rabbits using testchemical 2 -Picoline (CAS no.: 109 -06 -8, E.C. no.: 203 -643 -7). Rabbits were treated with the undiluted test chemical with and without washing. Animals were further observed for clinical signs of irritation. There was slight pain upon direct contact followed by severe conjunctival and corneal injury. The test substance was severely irritating to rabbit eye. Hence, the test chemical can be considered as "Category 2" according to the CLP.

2. The ocular irritation potential of 3-methylpyridine was assessed in rabbits. Undiluted 3-methylpyridine (CAS no.108 -99 -6, E.C. no.: 203 -636 -9)was applied to the eyes of male New Zealand albino rabbits and observed for signs of irritation till 7 or 14 days.Following 7 or 14 days rabbits were killed and histological examinations were performed on the eyes. Eye irritation was characterized by ocular irritation indices (OII). Eye irritation assessments showed that irritation was maximal either 1 hr or 1 day after exposure. Histological examination of the eyes showed keratitis lesions in corneal epithelial tissue, fibrillary edematous lamallae dissociation, and cellular inflammatory infiltration. Over longer periods of time, which included recovery, 3-methylpyridine was considered to be severely irritating to rabbit eyes and can be considered under "Category 2", as per the CLP criteria.

Justification for classification or non-classification

The skin and eye irritation potential of test chemical was observed in various studies. The results obtained from these studies indicate that the chemical is likely to cause skin and eye irritation. Hence the test chemical can be classified as "Category 2" irritant as per CLP.