2,6-dimethylpyridine

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Remarks:

Read across data

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from secondary literature

Data source

Reference

Reference Type:

other: Secondary literature

Title:

in vitro bacterial gene mutation assay of 2-Picoline.

Author:

U. S. Environmental Protection Agency (EPA) Office of Pollution Prevention and Toxic Substances (OPPTS)

Year:

2009

Bibliographic source:

Hazard Characterization - Pyridine and Pyridine Derivatives Category, U. S. Environmental Protection Agency (EPA) Office of Pollution Prevention and Toxic Substances (OPPTS), 2009

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2-methylpyridine

EC Number:

203-643-7

EC Name:

2-methylpyridine

Cas Number:

109-06-8

Molecular formula:

C6H7N

IUPAC Name:

2-methylpyridine

Test material form:

liquid

Details on test material:

Substance name: 2-Picoline
CAS no.: 109-06-8
Mol. wt.: 93.13
Smiles: CC1=CC=CC=N1
Physicl state: Liquid
Purity: Not specified

Method

Target gene:

Histidine gene

Species / strain

Species / strain / cell type:

other: TA97, TA98, TA100, TA102, TA 1535 and/or TA 1537

Details on mammalian cell type (if applicable):

Not specified

Metabolic activation:

with and without

Metabolic activation system:

Not specified

Test concentrations with justification for top dose:

up to 5000 µg/plate

Vehicle / solvent:

Not specified

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

not specified

Details on test system and experimental conditions:

Not specified

Evaluation criteria:

Not specified

Statistics:

Not specified

Results and discussion

Test results

Species / strain:

other: TA97, TA98, TA100, TA102, TA 1535 and/or TA 1537

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

not specified

Untreated negative controls validity:

valid

True negative controls validity:

not specified

Positive controls validity:

valid

Remarks on result:

other: Non-mutagenic

Applicant's summary and conclusion

Conclusions:

The test substance did not show increase in the number of revertant colonies. Hence, the test susbstance is considered to be non-mutagenic.

Executive summary:

in vitro Gene mutation toxicity study was performed to determine the mutagenic nature of the read across substance 2 -Picoline (CAS no.: 109 -06 -8, E.C. no.: 203 -643 -7). The study was performed using Salmonella typhimurium strains TA97, TA98, TA100, TA102, TA1535 and/or TA1537 in the presence and absence of metabolic activation system. The chemical was used at dose levels upto 5000 µg/plate. Positive and negative controls were tested concurrently. The test chemical did not induce gene mutation in Salmonella typhimurium strains in the presence and absence of metabolic activation system and hence the chemical is not likely to classify as a gene mutant in vitro.