L-Aspartic acid, N,N'-1,2-ethanediylbis-, sodium salt (1:3)

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: long-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From Sep. 30 to Nov. 6, 1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

not specified

Details on sampling:

Not applicable

Vehicle:

yes

Details on preparation and application of test substrate:

- Method of mixing into soil (if used): mechanical mixing
- Controls: vehicle control
- Chemical name of vehicle: deionized water
- Concentration of vehicle in test medium: stock solution: 130.1, 410.1, 1270.4, 4045.1 and 12,632.2 mg/L

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: Earthworm
- Source: Commercial hatchery Blades Biological, Crowden-Edenbridge, Kent, England
- Weight at test initiation (mean and range, SD): 398-599 mg


ACCLIMATION
- Acclimation conditions: Worms were cultured in the laboratory under standard conditions in a horse-manure, garden soil mixture (1:1) at about 24°C for 20 days prior to the main study

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Post exposure observation period:

None

Test temperature:

20.1-20.8°C

pH:

5.9-6.3

Moisture:

53.8-54.2%

Details on test conditions:

TEST SYSTEM
- Test container (material, size): glass jars (1 L), provided with a glass lid (domestic preserving jars)
- Amount of soil or substrate: 775 g wet weight
- No. of organisms per container (treatment): ten
- No. of replicates per treatment group: four
- No. of replicates per vehicle control: four


SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): artificial soil consisted of finely ground sphagnum peat (no visible plant remains), kaolin clay and fine industrial sand at a ratio of 1:2:7 (based on dry weight) respectively.
- Source: Sphagnum peat: Delft, The Netherlands; fine industrial sand: Vreeswijk, The Netherlands; kaolin clay: Haastrecht, The Netherlands


OTHER TEST CONDITIONS
- Photoperiod: continuous light
- Light intensity: 400-800 lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Survival: observed on Day 7 and 14
- Condition (appearance and behaviour) of worm: observed on Day 0, 7 and 14
- Weight of worms: observed on Day 0 and 14
- pH and moisture content: observed on Day 0 and 14
- Temperature and light intensity: observed on Day 0, 7 and 14

VEHICLE CONTROL PERFORMED: Yes


TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 3.2
- Range finding study: doses in main test were based on the adverse effects observed in range finding test

Nominal and measured concentrations:

0, 10, 32, 100, 320 and 1000 mg/kg dry soil (nominal)

Reference substance (positive control):

no

Duration:

7 d

Dose descriptor:

LC50

Effect conc.:

128 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 101-163

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

115 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 90.4-145

Duration:

14 d

Dose descriptor:

LC0

Effect conc.:

10 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

14 d

Dose descriptor:

LC100

Effect conc.:

1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

32 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

32 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: behaviour, appearance and weight

Details on results:

- Mortality at end of exposure period (Day 14) were 0/40, 0/40, 1/40, 21/40, 34/40 or 40/40 at 0, 10, 32, 100, 320 or 1000 mg/kg dry soil, respectively
- Average weight at begining of test (Day 0): 500, 494, 503, 503, 491 or 500 mg at 0, 10, 32, 100, 320 or 1000 mg/kg dry soil, respectively
- Average % weight change: (loss) 10.5 ± 2.0, 10.4 ± 3.3, 14.6 ± 5.8, 24.7 ± 7.4, 20.4 ± 4.7 or 100 ± 0 at 0, 10, 32, 100, 320 or 1000 mg/kg dry soil, respectively
- Behavioural abnormalities: surviving worms were observed to be slower at 100 and 320 mg/kg dry soil

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

- NOEC for weight: Analysed by two-tailed Dunnett-test
- NOEC for survival: Analysed by binomial test

Validity criteria fulfilled:

yes

Conclusions:

In a GLP study conducted according to OECD Guideline 207, 14-d LC50, LC0 and LC100 (survival) of trisodium EDDS to the earthworm, Eisenia fetida, were calculated to be 115, 10 and 1000 mg/kg dry soil, respectively. 

Further, 14-d NOEC for survival, appearance, behaviour and weight was 32 mg/kg dry soil.

Executive summary:

A GLP study was conducted according to OECD Guideline 207 to determine the short-term toxicity of trisodium EDDS to the earthworm, Eisenia fetida. Worms were exposed to trisodium EDDS (in water), mixed with an artificial soil, at nominal concentrations of 0, 10, 32, 100, 320 and 1000 mg/kg dry soil, and observed for survival, appearance and behaviour after 7 and 14 days of exposure.

Mortality at the end of exposure period (Day 14) was 0/40, 0/40, 1/40, 21/40, 34/40 or 40/40 at 0, 10, 32, 100, 320 or 1000 mg/kg dry soil, respectively. Survival was statistically significantly reduced compared to controls at 100 mg/kg dry soil and above. Surviving worms were observed to be slower at concentrations of 100 and 320 mg/kg dry soil. Average weight losses were 10.5, 10.4, 14.6, 24.7, 20.4 or 100% at 0, 10, 32, 100, 320 or 1000 mg/kg dry soil, respectively.

In conclusion, 14-d LC50, LC0 and LC100 of trisodium EDDS to Eisenia fetida were calculated to be 115, 10 and 1000 mg/kg dry soil, respectively. Further, 14-d NOEC for survival, appearance, behaviour and weight was 32 mg/kg dry soil.

Description of key information

In a GLP study conducted according to OECD Guideline 207, the 14-d LC50, LC0 and LC100 (survival) of trisodium EDDS to the earthworm, Eisenia fetida, were calculated to be 115, 10 and 1000 mg/kg dry soil, respectively. 

Further, the 14-d NOEC for survival, appearance, behaviour and weight loss was 32 mg/kg dry soil.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

115 mg/kg soil dw

Additional information

A GLP study was conducted according to OECD Guideline 207 to determine the short-term toxicity of trisodium EDDS to the earthworm, Eisenia fetida. Worms were exposed to trisodium EDDS (in water), mixed with an artificial soil, at nominal concentrations of 0, 10, 32, 100, 320 and 1000 mg/kg dry soil, and observed for survival, appearance and behaviour after 7 and 14 days of exposure. Mortality at the end of exposure period (Day 14) was 0/40, 0/40, 1/40, 21/40, 34/40 or 40/40 at 0, 10, 32, 100, 320 or 1000 mg/kg dry soil, respectively. Survival was statistically significantly reduced compared to controls at 100 mg/kg dry soil and above. Surviving worms were observed to be slower at concentrations of 100 and 320 mg/kg dry soil. Average weight losses were 10.5, 10.4, 14.6, 24.7, 20.4 or 100% at 0, 10, 32, 100, 320 or 1000 mg/kg dry soil, respectively. In conclusion, 14-d LC50, LC0 and LC100 of trisodium EDDS to Eisenia fetida were calculated to be 115, 10 and 1000 mg/kg dry soil, respectively. Further, the 14-d NOEC for survival, appearance, behaviour and weight loss was 32 mg/kg dry soil (Henzen, 1999a).