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EC number: 416-530-4 | CAS number: 178949-82-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 02.04.93 - 07.04.93
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Principles of method if other than guideline:
- Adsorption coefficients were determined for the partitioning of the test substance at two different concentrations between an aqueous phase and a known mass of activated sewage sludge either pre-exposed to the test substance (adapted) or not (nonadapted). Inactivation (sterilisation) of incubations containing adapted sludge was attempted using sodium azide (Na3N) to prevent biodegradative losses of the test substance during equilibration. However, Na3N was not an effective sterilant with respect to microorganisms capable of degrading the test substance and as such biodegradative losses of the test substance from these incubations resulted in low recoveries and inaccurate determinations of adsorption coefficients. The ineffectiveness of Na3N to inactivate the adapted sludge was assessed in a parallel study in which biodegradative mineralisation of radiolabelled test substance was quantified by liquid scintillation counting (LSC) of evolved 14C-CO2 trapped in ethanolamine.
Adapted and nonadapted sludge which had been incubated with the test substance during the adsorption tests was subsequently used to determine coefficents of desorption.
Liquid-solid partitioning in adsorption and desorption tests was assessed using a mixture of radiolabelled and non-radiolabelled test substance with quantification of test substance concentrations of both the liquid phase and sludge suspended solid phase by LSC.
A preliminary study (equivalent to test controls containing no sludge) concluded adsorption of the test compound to labware (e.g. glass vessels used for incubations, pipettes etc) was insignificant.
Methodological weaknesses arise from the following:
- no report of tests undertaken to establish equilibration time between test substance and sludge
- no report of pH measurment or control
- no report of blank tests (i.e. sludge + aqueous phase only)
- no report of control of ionic strength of aqueous phase
- no report of temperature control - GLP compliance:
- yes
- Type of method:
- batch equilibrium method
- Media:
- sewage sludge
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
no data - Radiolabelling:
- yes
- Test temperature:
- no data
- Details on study design: HPLC method:
- not applicable
- Analytical monitoring:
- yes
- Details on sampling:
- Adsorption tests:
1. Centrifugation of 100 mL test substance/sludge after 3 h incubation.
2. 2 x 2 mL supernatent samples taken by pipette and added to 17 mL Lumagel (scintillation cocktail) for liquid scintillation counting (LSC).
3. Supernatent discarded and sludge solids resuspended in 100 mL water.
4. 2 x 1 mL suspended sludge solids samples taken by pipette and added to 9 mL deionised water and 10 mL Lumagel for LSC analysis.
Desorption tests:
5. Centrifugation of 100 mL water/sludge after 3 hrs incubation.
6. 2x 2 mL supernatant samples taken by pipette and added to 17 mL Lumagel (scintillation fluid) for LSC.
7. Supernatent discarded and sludge solids resuspended in 100 mL water.
8. 2 x 1 mL suspended sludge solids samples taken by pipette and added to 9 mL deionised water and 10 mL Lumagel for LSC analysis. - Details on matrix:
- - Type of sludge: activated domestic sewage sludge either pre-exposed or non-exposed to the test substance.
- Source of sludge: adapted sludge: obtained from previous CAS- test ECM ETS 465/03; non-adapted sludge: obtained from Bocholt Waste Water Treatement Works, Belgium.
- Pretreatment: none reported
PREPARATION OF SLUDGE SOLIDS
- Separation from wastewater: no data
- Further preparation procedures: no data
- Details of lyophilization: not performed
- Details of desiccation: not performed - Details on test conditions:
- PRELIMINARY STUDY
To assess losses of test substance to test vessels (glass centrifuge tube) and pipette tips:
Preliminary vessels 1 and 2:
100 mL of 0.1 mg/L non-radiolabelled test substance
+ 0.050 mL radiolabelled test substance stock solution
The test container was rinsed with 3 x 10 mL of methanol to give a total rinse volume of 30 mL from which 10 mL was analysed by LSC.
The pipette tip was rinsed with 3 x 1 mL methanol and the 3 mL total rinse volume was analysed by LSC.
TEST CONDITIONS
- Buffer: none
- pH: not reported
- Suspended solids concentration: determined at 105 oC: adapted sludge = 1.89 g/L and non-adapted sludge = 2.35 g/L; determined at 550 oC: adapted sludge = 1.53 g/L and non-adapted sludge = 1.92 g/L
TEST SYSTEM
- Type, size and further details on reaction vessel: 200 mL glass centrifuge tube
- Water filtered (i.e. yes/no; type of size of filter used, if any): no data
- Amount of sludge and water per treatment: 100 mL
- Measuring equipment: LSC
- Test performed in closed vessels due to significant volatility of test substance: no data
- Test performed in open system: no data
- Method of agitation: continuous aeration
- Method of preparation of test solution:
The test solutions were prepared by mixing the appropriate volumes of activated sludge, radiolabelled test substance, stock solution of non-labelled test substance and sodium azlde solution (20 mg/mL) as per the following:
vessels 1 and 2:
100 mL non-adapted activated sludge
+ 0.050 mL radiolabelled test substance stock solution
+ 0.1 mL non-radiolabelled test sustance stock solution
vessels 3 and 4:
100 ml non-adapted activated sludge
+ 0.050 mL radiolabelled test susbtance stock solution
+ 1.0 mL stock solution of the non-labelled test substance stock solution
vessels 5 and 6:
100 ml adapted activated sludge
+ 0.050 mL radiolabelled test substance stock solution
+ 0.1 mL non-radiolabelled test substance stock solution
+ 1 mL sodium azide solution
vessels 7 and 8:
100 mL activated sludge
+ 0.050 mL labeled test matertal
+1.000 mL stock solution of the non-labeled test matertal
+ 1 mL sodium azide solution
- Are the residues from the adsorption phase used for desorption: yes. After centrifugation and sampling, supernatants from adsorption tests were discarded and vessels refilled with 100 mL water. After 3 hours incubation, desorption coefficients were determined by analysis of aqueous and soilid phases for test substance. - Computational methods:
- Sorption Coefficient
Kd = Concentration of test material on solids/Concentration of test materials in water = (DPM on solids/DPM in water) x (Wt of water/Wt of solids) - Key result
- Sample No.:
- #1
- Type:
- Kd
- Value:
- 388 dimensionless
- % Org. carbon:
- 100
- Sample No.:
- #2
- Type:
- Kd
- Value:
- 455 dimensionless
- % Org. carbon:
- 100
- Sample No.:
- #3
- Type:
- Kd
- Value:
- 372 dimensionless
- % Org. carbon:
- 100
- Sample No.:
- #4
- Type:
- Kd
- Value:
- 295 dimensionless
- % Org. carbon:
- 100
- Details on results (HPLC method):
- not applicable
- Adsorption and desorption constants:
- Adsorption coefficients (Kd abs)
Vessels 1 & 2 (0.1 mg/L test substance + non-adapted sludge): mean Kd abs = 40
Vessels 3 & 4 (1.0 mg/L test substance + non-adapted sludge): mean Kd abs = 37
Vessels 5 & 6 (0.1 mg/L test substance + adapted sludge): mean Kd abs = 71 ((low mass balance (see Table 2) indicates biodegradative loss of test substance and inaccurate adsorption coefficient)
Vessels 7 & 8 (1.0 mg/L test substance + adapted sludge): mean Kd abs = 90 (low mass balance (see Table 2) indicates biodegradative loss of test substance and inaccurate adsorption coefficient)
Desorption coefficients (Kd des)
Vessels 1 & 2 (0.1 mg/L test substance + non-adapted sludge): mean Kd des = 421
Vessels 3 & 4 (1.0 mg/L test substance + non-adapted sludge): mean Kd des = 334
Vessels 5 & 6 (0.1 mg/L test substance + adapted sludge): mean Kd des = 1034 ((low mass balance (see Table 2) indicates biodegradative loss of test substance giving inaccurate desorption coefficient)
Vessels 7 & 8 (1.0 mg/L test substance + adapted sludge): mean Kd des = 1298 (low mass balance (see Table 2) indicates biodegradative loss of test substance giving inaccurate desorption coefficient)
Also See Table 2 - Recovery of test material:
- See Table 2
- Concentration of test substance at end of adsorption equilibration period:
- See Table 2
- Concentration of test substance at end of desorption equilibration period:
- See Table 2
- Transformation products:
- not specified
- Details on results (Batch equilibrium method):
- - Preliminary tests assessed adsorption of the test substance onto labware to be insignificant (see Table 1).
- Losses of test substance from vessels 5 to 8 in which the test substance was incubated with adapted sludge were attributed to biodegradation. The adsorption coefficients determined for these incubations were therefore unreliable (see Table 2). - Validity criteria fulfilled:
- not applicable
- Conclusions:
- In a GLP study, adsorption of trisodium EDDS to activated sludge was considered minimal.
- Executive summary:
In a GLP study, adsorption (Kd abs) and desorption (Kd des) coefficients were determined (using the batch equilibrium method) for incubations of two different concentrations (0.1 and 1.0 mg/L) of trisodium EDDS with activated domestic sewage sludge that had either been pre-exposed (adapted) to the test substance or not (non-adapted). The addition of sodium azide to incubations with adapted sludge was ineffective in preventing the biodegradation of the test substance and therefore Kd abs and Kd des values derived from these tests were considered unreliable. Incubations of the test substance at concentrations of 0.1 and 1.0 mg/L with non-adapted sludge yielded K abs values of 40 and 37, respectively. Corresponding Kd des values of 421 and 334 were determined using the sludge residues from these adsorption tests. A 14C-radiolabelled test substance tracer was used to assess partitioning between the aqueous and suspended solid phases of the sludge. A preliminary study determined the adsorption of test substance, trisodium EDDS, to labware (i.e. glass test vessel and pipette tips) to be insignificant.
From the Kd abs and Kd des values derived, it was concluded that adsorption of trisodium -EDDS to activated sewage sludge was minimal.
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- From May 24, to June 01, 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 106 (Adsorption - Desorption Using a Batch Equilibrium Method)
- Deviations:
- yes
- Remarks:
- See section 'Any other information on materials and methods'
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- batch equilibrium method
- Media:
- soil
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No data - Radiolabelling:
- no
- Test temperature:
- Room temperature
- Details on study design: HPLC method:
- Not applicable
- Analytical monitoring:
- yes
- Details on sampling:
- Concentrations of test sample: 25.1 mg/L in 0.01 M CaCl2
- Details on matrix:
- Soil I (Samples #1 and #2):
COLLECTION AND STORAGE
- Geographic location: Wageningen, The Netherlands.
- Collection procedures: no data
- Sampling depth (cm): no data
- Storage conditions: no data
- Storage length: no data
- Soil preparation (e.g.: 2 mm sieved; air dried etc.): 2 mm sieved and air dried
PROPERTIES
- Soil texture
- % sand: 81.3
- % silt: 11.1
- % clay: 6.0
- Horizon: 0-30 cm
- Soil taxonomic classification: strong silty sand
- Soil classification system: no data
- Soil series: no data
- Soil order: no data
- pH: 4.0
- Dry weight content (%) - 99.6
- Organic carbon (%): 1.4
- CEC (meq/100 g): 4.7
- Carbonate as CaCO3: 0.2 %
- Insoluble carbonates (%): no data
- Extractable Cations (Ca, Mg, Na, K, H) (MEQ/100 g): Ca: 1.33; Mg: 0.17; Na: <0.01; K: 0.23; H: 5.5
- Special chemical/mineralogical features: no data
- Clay fraction mineralogy: no data
- Moisture at 1/3 atm (%): no data
- Bulk density (g/cm3): no data
- Biomass (e.g. in mg microbial C/100 mg, CFU or other): no data
Soil II (Samples #3 and #4):
COLLECTION AND STORAGE
- Geographic location: Kloosterburen, The Netherlands.
Collection procedures: no data
- Sampling depth (cm): no data
- Storage conditions: no data
- Storage length: no data
- Soil preparation (e.g.: 2 mm sieved; air dried etc.): 2 mm sieved and air dried
PROPERTIES
- Soil texture
- % sand: 41.7
- % silt: 39.8
- % clay: 13.6
- Horizon: 0-30 cm
- Soil taxonomic classification: strong sandy loam
- Soil classification system: no data
- Soil series: no data
- Soil order: no data
- pH: 7.5
- Dry weight content (%) - 99.9
- Organic carbon (%): 1.8
- CEC (meq/100 g): 10.9
- Carbonate as CaCO3: 3.1 %
- Insoluble carbonates (%): no data
- Extractable Cations (Ca, Mg, Na, K, H) (MEQ/100 g): Ca: 9.06; Mg: 1.0; Na: 0.15; K: 0.96; H: 0.6
- Special chemical/mineralogical features: no data
- Clay fraction mineralogy: no data
- Moisture at 1/3 atm (%): no data
- Bulk density (g/cm3): no data
- Biomass (e.g. in mg microbial C/100 mg, CFU or other): no data
Soil III (Samples #5 and #6):
COLLECTION AND STORAGE
- Geographic location: Beek, The Netherlands.
- Collection procedures: no data
- Sampling depth (cm): no data
- Storage conditions: no data
- Storage length: no data
- Soil preparation (e.g.: 2 mm sieved; air dried etc.): 2 mm sieved and air dried
PROPERTIES
- Soil texture
- % sand: 11.9
- % silt: 74.7
- % clay: 12.1
- Horizon: 0-30 cm
- Soil taxonomic classification: weak sandy loam
- Soil classification system: no data
- Soil series: no data
- Soil order: no data
- pH: 6.6
- Dry weight content (%) - 99.9
- Organic carbon (%): 1.1
- CEC (meq/100 g): 7.5
- Carbonate as CaCO3: 0.2%
- Insoluble carbonates (%): no data
- Extractable Cations (Ca, Mg, Na, K, H) (MEQ/100 g): Ca: 5.93; Mg: 0.61; Na: 0.22; K: 0.17; H: 1.0
- Special chemical/mineralogical features: no data
- Clay fraction mineralogy: no data
- Moisture at 1/3 atm (%): no data
- bulk density (g/cm3): no data
- Biomass (e.g. in mg microbial C/100 mg, CFU or other): no data - Details on test conditions:
- TEST CONDITIONS
Three soil types were tested (Soil I, Soil II and Soil III). per soil type, triplicate 5 g samples of soil were pre-equilibrated/washed with 10 mL of distilled water in 50 mL glass vials for 5 days at room temperature with continuous agitation by tumbling. Thereafter, vials were centrifuged for 10 minutes at 170 g and 20oC and most of the supernatants removed. Adsorption/desorption screening was performed on the soil precipitates at room temperature. A 16 h equilibration time was used in adsorption and desorption testing. Soil residues used in adsorption testing were used.
TEST SYSTEM
- Buffer: no buffers used
- pH: pH of the test soils was measured prior to equilibration with test substance (method of pH measurment not reported). pH of test vessels (soil + test substance solution) was not measured.
- Suspended solids concentration: 5 g air dried soil
Adsorption screening test:
- Preparation of test solution: test substance dissolved in 0.01 M CaCl2
- Reaction vessel: 50 mL glass vial
- Water filtered: no data
- Amount of soil/water per treatment: 5 g soil (air-dried weight) per 25 mL test substance solution (25.1 test substance in 0.01 M CaCl2)
- Soil/solution ratio: 1:5
- Reaction vessels/concentration: per soil type (i.e. soil I, II and III) duplicate vessels contained 5 g soil (air-dried weight) and 25 mL of 25.1 mg/L test substance in 0.01 M CaCl2. Also per soil type a singular blank contained 5 g soil (air-dried weight) and 25 mL of 0.01 M CaCl2. A singular control contained 25 mL of 25.1 mg/L test substance in 0.01 M CaCl2 only to assess loss of the test substance due to adsorption to the interior of the vessel.
- Measuring equipment: for the liquid phase (i.e. test substance and CaCl2 solutions) quantities were determined gravimetrically. pH measurement prior to and after equilibration of test substance with soil was not reported.
- Test performed in closed vessels for agitation by continuous tumbling.
- Equilibration time: 16 h
- Cetrifugation: test vessels centrifuged for 10 minutes at 170 x g and 20oC for separation of soil and solution phases.
- Supernatants analysed by HPLC for test substance concentration.
- Soil residues from adsorption tests were used for desorption tests.
Desorption screening test:
- Reaction vessel: same 50 mL glass vial used in adsorption tests.
- Amount of soil/water per treatment: same 5 g soil (air-dried weight) used in adsorption tests per 25 mL 0.01 M CalCl2.
- Soil/solution ratio: 1:5
- Extractions: two sequential extractions
- Cetrifugation: test vessels centrifuged for 10 minutes at 170 x g and 20 oC for separation of soil and solution phases.
- Supernatents analysed by HPLC for test substance concentration. - Sample No.:
- #1
- Duration:
- 16 h
- Initial conc. measured:
- 130.97 mg/kg soil d.w.
- Sample No.:
- #2
- Duration:
- 16 h
- Initial conc. measured:
- 135.84 mg/kg soil d.w.
- Sample No.:
- #3
- Duration:
- 16 h
- Initial conc. measured:
- 142.47 mg/kg soil d.w.
- Sample No.:
- #4
- Duration:
- 16 h
- Initial conc. measured:
- 142.82 mg/kg soil d.w.
- Sample No.:
- #5
- Duration:
- 16 h
- Initial conc. measured:
- 135.39 mg/kg soil d.w.
- Sample No.:
- #6
- Duration:
- 16 h
- Initial conc. measured:
- 137.05 mg/kg soil d.w.
- Sample no.:
- #1
- Duration:
- 32 h
- Conc. of adsorbed test mat.:
- 128.35 mg/kg soil d.w.
- Sample no.:
- #2
- Duration:
- 32 h
- Conc. of adsorbed test mat.:
- 133.12 mg/kg soil d.w.
- Sample no.:
- #3
- Duration:
- 32 h
- Conc. of adsorbed test mat.:
- 117.97 mg/kg soil d.w.
- Sample no.:
- #4
- Duration:
- 32 h
- Conc. of adsorbed test mat.:
- 119.83 mg/kg soil d.w.
- Sample no.:
- #5
- Duration:
- 32 h
- Conc. of adsorbed test mat.:
- 121.04 mg/kg soil d.w.
- Sample no.:
- #6
- Duration:
- 32 h
- Conc. of adsorbed test mat.:
- 121.56 mg/kg soil d.w.
- Computational methods:
- Adsorption screening test:
The percent of the test substance adsorbed was calculated as:
A = 100 * (G - (Ce * Vo))/G = 100 * (X/G)
The adsorption coefficient for the determination was calculated as:
K' =( X/M)/Ce
The adsorption coefficient was also calculated as a function of the organic carbon content of the soil:
K'oc =. (K'/% organic carbon) * 100
Desorption screening test:
The percent of the adsorbed test substance which was desorbed was calculated as:
D=100 x ((((Cl + C2) * V) - ((Vo - V) * Ce))/X)
The percent of the adsorbed test substance which was not desorbed was calculated as:
R= 100 x ((G - (Ce + Cl + C2) * V)/X)
where,
G = quantity of materlal employed (ug) (corrected for the recovery of the blank without soil)
Ce = concentration of test substance remaining in the solution after adsorption (ug/mL)
Vo = original volume of solution employed (mL)
M = dry weight of soil employed (g)
C1 = concentration of test substance in the solution after the first wash (ug/mL)
C2 = concentration of test substance in the solution after the second wash (ug/mL)
V = voIume of solution obtained after the adsorption step (mL)
X = amount of test substance adsorbed (ug) - Type:
- Kd
- Remarks:
- Sample #1 (Soil I)
- Value:
- 253
- % Org. carbon:
- 1.4
- Remarks on result:
- other: room temperature
- Type:
- Kd
- Remarks:
- Sample #2 (Soil I)
- Value:
- 264
- % Org. carbon:
- 1.4
- Remarks on result:
- other: room temperature
- Type:
- Kd
- Remarks:
- Sample #3 (Soil II)
- Value:
- 27.3
- % Org. carbon:
- 1.8
- Remarks on result:
- other: room temperature
- Type:
- Kd
- Remarks:
- Sample #4 (Soil II)
- Value:
- 29.5
- % Org. carbon:
- 1.8
- Remarks on result:
- other: room temperature
- Type:
- Kd
- Remarks:
- Sample #5 (Soil III)
- Value:
- 45.6
- % Org. carbon:
- 1.1
- Remarks on result:
- other: room temperature
- Type:
- Kd
- Remarks:
- Sample #6 (Soil III)
- Value:
- 42.3
- % Org. carbon:
- 1.1
- Remarks on result:
- other: room temperature
- Adsorption and desorption constants:
- Sample # Soil Kd Koc
1 I 253 18100
2 I 264 18900
3 II 27.3 1510
4 II 29.5 1640
5 III 45.6 4150
6 III 42.3 3850 - Concentration of test substance at end of adsorption equilibration period:
- See Table 2
- Concentration of test substance at end of desorption equilibration period:
- See Table 2
- Sample no.:
- #1
- Duration:
- 16 h
- % Adsorption:
- 98
- Sample no.:
- #2
- Duration:
- 16 h
- % Adsorption:
- 98
- Sample no.:
- #3
- Duration:
- 16 h
- % Adsorption:
- 82.8
- Sample no.:
- #4
- Duration:
- 16 h
- % Adsorption:
- 83.9
- Sample no.:
- #5
- Duration:
- 16 h
- % Adsorption:
- 89.4
- Sample no.:
- #6
- Duration:
- 16 h
- % Adsorption:
- 88.7
- Sample no.:
- #1
- Duration:
- 32 h
- % Desorption:
- < 3.65
- Sample no.:
- #2
- Duration:
- 32 h
- % Desorption:
- < 3.64
- Sample no.:
- #3
- Duration:
- 32 h
- % Desorption:
- < 3.72
- Sample no.:
- #4
- Duration:
- 32 h
- % Desorption:
- < 3.25
- Sample no.:
- #5
- Duration:
- 32 h
- % Desorption:
- < 4
- Sample no.:
- #6
- Duration:
- 32 h
- % Desorption:
- < 3.58
- Transformation products:
- not measured
- Details on results (Batch equilibrium method):
- See Table 2
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a GLP study similar to OECD Guideline 106, the amount of adsorption (A) of trisodium EDDS was determined to be 98%, 83% and 89% for Soil I, II and III, respectively, and the corresponding desorption (D) was determined to be <4%, <3% and <4%. Therefore, under the conditions of this test, it can be concluded that trisodium EDDS has a high affinity for soil.
- Executive summary:
A batch equilibrium screening study was conducted to assess adsorption/desorption of trisodium-EDDS with three different soils. The study methology was based on OECD Guideline 106 available at the time (1981), however the study has several deviations from the current version adopted in 2000. The test soils were - Soil I; Strong silty sand (pH 4.0, 6% clay, 1.4% organic matter); Soil II: Strong sandy loam (pH 7.5, 13.6% clay, 1.8% organic matter); Soil III; Weak sandy loam (pH 6.6, 12.1% clay, 1.1% organic matter).
The test substance had high affinity for the test soils with adsorption (A) determined as 98%, 83% and 89% for soil I, II and III, respectively, with corresponding Kd values of 260, 28 and 44. Desorption (D) of the test substance from the soil was low with values determined as <4%, <3% and <4% for soil I, II and III respectively. The amount of adsorbed material which was not desorbed (R) was determined to be >96%, >97% and >96% for Soil I, II and III, respectively. The corresponding K' values were calculated to be 2.6 x 102, 28 and 44 for Soil I, II and III, respectively, and Koc values of about 1.9 x 104, 1.6 x 103 and 4 x 103.
Therefore, under the conditions of this test, it can be concluded that trisodium EDDS has a high affinity for soil.
Referenceopen allclose all
Table 1:
|
Preliminary vessel 1 |
Preliminary vessel 2 |
|
test substance concentration (mg/L) |
0.1 |
0.1 |
|
t0spike (dpm) |
214382 |
214382 |
|
supernatant |
dpm |
218370 |
208677 |
% of t0 |
101.86 |
97.34 |
|
test vessel rinses |
dpm |
1035 |
695 |
% of t0 |
0.48 |
0.32 |
|
pipette tip rinses |
dpm |
35 |
22 |
% of t0 |
0.02 |
0.01 |
|
% recovery |
102 |
98 |
Table 2: LSC measurements for the adsorption/desorption test and calculations of the Kd values.
Vessel # | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
t0-spike (dpm) | 214382 | 214382 | 214382 | 214382 | 214382 | 214382 | 214382 | 214382 |
ADSORPTION | ||||||||
dpm on solids (dpm/g) | 79894 | 82011 | 74603 | 75661 | 71489 | 75745 | 80000 | 81702 |
dpm In water (dpm/g) | 1953 | 2055 | 1978 | 2086 | 1262 | 894 | 1146 | 740 |
Kd abs | 41 | 40 | 38 | 36 | 57 | 85 | 70 | 110 |
% recovery | 98 | 103 | 99 | 104 | 67 | 50 | 62 | 43 |
DESORPTION | ||||||||
dpm on solids (dpm/g) | 46561 | 48677 | 37566 | 35450 | 47660 | 62979 | 50638 | 63830 |
dpm In water (dpm/g) | 120 | 107 | 101 | 120 | 63 | 48 | 43 | 45 |
Kd des | 388 | 455 | 372 | 295 | 757 | 1312 | 1178 | 1418 |
% recovery | 138 | 128 | 122 | 131 | 104 | no | 86 | 102 |
NOTE: Adsorption coefficents (Kd abs) and desorption coefficients (Kd des) for vessels 5 to 8 are not reliable due to biodegradative losses of the test compound.
Table 2: Measurements of the screening adsorption/desorption test of trisodium EDDS at room temperature
Sample # |
1 |
2 |
3 |
4 |
5 |
6 |
Soil |
I |
I |
II |
II |
III |
III |
G (µg) |
518 |
542 |
528 |
529 |
528 |
533 |
Ce (µg/mL) |
0.4 |
0.4 |
3.2 |
3.0 |
2.1 |
2.2 |
V0 (mL) |
26.09 |
27.06 |
28.38 |
28.45 |
26.97 |
27.30 |
M (g) |
5.02 |
5.03 |
5.02 |
5.01 |
5.01 |
5.05 |
A (%) |
98.0 |
98.0 |
82.8 |
83.9 |
89.4 |
88.7 |
Kd |
253 |
264 |
27.3 |
29.5 |
45.6 |
42.3 |
Koc |
18100 |
18900 |
1510 |
1640 |
4150 |
3850 |
V (mL) |
24.09 |
25.11 |
24.82 |
24.82 |
24.68 |
24.97 |
C1 (µg/mL) |
<0.4 |
<0.4 |
0.71 |
0.62 |
0.56 |
0.49 |
C2 (µg/mL) |
<0.4 |
<0.4 |
<0.4 |
<0.4 |
<0.4 |
<0.4 |
D (%) |
<3.65 |
<3.64 |
<3.72 |
<3.25 |
<4.00 |
<3.58 |
R (%) |
>96.4 |
>96.4 |
>96.3 |
>96.8 |
>96.0 |
>96.4 |
G ¿ quantity of test substance employed (corrected for the recovery of the blank without soil)
Ce ¿ concentration of test substance remaining in solution after adsorption
V0 ¿ original volume of test substance solution employed
M ¿ dry weight of soil employed
A ¿ percentage of test substance adsorbed
Kd ¿ adsorption coefficient
Koc¿ Kd as a function of organic carbon content
V ¿ volume of solution obtained after the adsorption step
C1 ¿ concentration of test substance in the solution after the first extraction/wash
C2 ¿ concentration of test substance in the solution after the second extraction/wash
D ¿ percentage of test substance desorbed from soil
R ¿ percentage of test substance not desorbed from soil
Table 3: Results of the screening adsorption/desorption test of trisodium EDDS at room temperature (average of duplicates of each test soil)
|
Soil I |
Soil II |
Soil III |
A[%] |
98 |
83 |
89 |
O[%] |
< 4 |
< 3 |
< 4 |
R [%] |
> 96 |
> 97 |
> 96 |
Kd |
2.6 x 102 |
28 |
44 |
Koc |
1.9 x 104 |
1.6 x 103 |
4.0 x 103 |
Description of key information
In reliable GLP studies (using the batch equilibrium method), adsorption of trisodium EDDS to activated sewage sludge was considered minimal (Geukens, 1993c), whereas the test substance had a high affinity for soil (de Vries, 1993d).
Key value for chemical safety assessment
Additional information
In a GLP study, adsorption (Kd abs) and desorption (Kd des) coefficients were determined (using the batch equilibrium method) for incubations of two different concentrations (0.1 and 1.0 mg/L) of trisodium EDDS with activated domestic sewage sludge that had not been pre-exposed to the test substance (non-adapted). Incubations of the test substance at concentrations of 0.1 and 1.0 mg/L with non-adapted sludge yielded K abs values of 40 and 37, respectively. Corresponding Kd des values of 421 and 334 were determined using the sludge residues from these adsorption tests. A 14C-radiolabelled test substance tracer was used to assess partitioning between the aqueous and suspended solid phases of the sludge. A preliminary study determined the adsorption of trisodium EDDS to labware (i.e. glass test vessel and pipette tips) to be insignificant. From the Kd abs and Kd des values derived, it was concluded that adsorption of trisodium EDDS to activated sewage sludge was minimal (Geukens, 1993c).
A batch equilibrium screening study was conducted to assess the adsorption/desorption of trisodium EDDS with three different soils. The study methodology was based on OECD Guideline 106 (1981) available at the time, however the study has several deviations from the current version adopted in 2000. The test soils were - Soil I; Strong silty sand (pH 4.0, 6% clay, 1.4% organic matter); Soil II: Strong sandy loam (pH 7.5, 13.6% clay, 1.8% organic matter); Soil III; Weak sandy loam (pH 6.6, 12.1% clay, 1.1% organic matter). The test substance had high affinity for the test soils with adsorption (A) determined as 98%, 83% and 89% for soil I, II and III, respectively, with corresponding Kd values of 260, 28 and 44. Desorption (D) of the test substance from the soil was low with values determined as <4%, <3% and <4% for soil I, II and III, respectively. The amount of adsorbed material which was not desorbed (R) was determined to be >96%, >97% and >96% for Soil I, II and III, respectively. The corresponding K' values were calculated to be 2.6 x 102, 28 and 44 for Soil I, II and III, respectively, and Koc values of about 1.9 x 104, 1.6 x 103 and 4 x 103 [log koc ranging from 3.6 to 4.28]. Therefore, under the conditions of this test, it can be concluded that trisodium EDDS has a high affinity for soil (de Vries, 1993d).
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