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EC number: 439-730-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 05 Jun 2003 to 04 Jul 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- GLP study performed according to OECD 305 and METI Guideline: Concentration Test on Chemical Substances in Fish
- Qualifier:
- according to guideline
- Guideline:
- other: METI Guideline: Concentration Test on Chemical Substances in Fish
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Radiolabelling:
- no
- Details on sampling:
- - Sampling intervals/frequency for test organisms: days 0, 7, 14, 21, 26, 28
- Sampling intervals/frequency for test medium samples: days 0, 7, 14, 21, 26, 28
- Details on sampling and analysis of test organisms and test media samples:
Water: Extraction with CH2Cl2 with further HPLC analysis.
Fish: two fish per sampling homogenised, and extracted with acetonitril/n-hexane. The analysis was performed using HPLC. - Vehicle:
- yes
- Details on preparation of test solutions, spiked fish food or sediment:
- In order to maintain the test material concentrations of 0.05 and 0.005 mg/L stable, Tween 80 was added as solubiliser at concentrations of 0.20 and 0.02 mg/L, respectively. The control contained Tween 80 at a concentration of 0.2 mg/L.
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- - Weight: 2.82 g
- Length: 6.4 cm
- Lot: 30407
- Acclimatisation: 07.04.2003 - 29.05.2003 - Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 28 d
- Total depuration duration:
- 0 d
- Hardness:
- 250 mg/L
- Test temperature:
- 24.6 ± 0.3 °C
- pH:
- 7.1
- Dissolved oxygen:
- 7.3 mg/L
- Salinity:
- According to guideline
- Details on test conditions:
- After acclimatisation period, the fish (Cyprinus carpio) were transferred into the test media under flow through conditions. Exposure concentrations were analytically verified at days 0, 7, 14, 21, 26 and 28. The analytical values show that the nominal concentrations of 0.05 and 0.005 mg/L were maintained at 98 and 100%, respectively. At days 0, 7, 14, 21, 26 and 28 two fish each were removed from each test group. Body weight was determined. The fish were homogenised and extracted with acetonitrile and n-hexane. The extract was analysed with HPLC. Due to the low bioaccumulation potential, no depuration phase was required.
- Nominal and measured concentrations:
- Nominal concentration 0.05 mg/L: analytically measured concentrations: day 0: 0.0480 mg/L; day 7: 0.0494 mg/L; day 14: 0.0496 mg/Lday 21: 0.0492 mg/Lday 26: 0.0499 mg/Lday 28: 0.0492 mg/L
nominal concentration 0.005 mg/L: analytically measured concentrations: day 0: 0.00509 mg/L; day 7: 0.00490 mg/L; day 14: 0.00492 mg/L; day 21: 0.00509 mg/L; day 26: 0.00494 mg/L; day 28: 0.00507 mg/L - Details on estimation of bioconcentration:
- according to guideline
- Key result
- Conc. / dose:
- 0.005 mg/L
- Temp.:
- 24.6 °C
- pH:
- 7.1
- Type:
- BCF
- Value:
- 180 dimensionless
- Basis:
- normalised lipid fraction
- Time of plateau:
- 21 d
- Calculation basis:
- steady state
- Key result
- Conc. / dose:
- 0.05 mg/L
- Temp.:
- 24.6 °C
- pH:
- 7.1
- Type:
- BCF
- Value:
- 145 dimensionless
- Basis:
- normalised lipid fraction
- Time of plateau:
- 21 d
- Calculation basis:
- steady state
- Details on kinetic parameters:
- Steady state was reached within 28 days. Due to the low uptake, no depuration phase was necessary.
- Metabolites:
- None.
- Details on results:
- - Mortality of test organisms: no effects
- Behavioural abnormalities: no effects
- Observations on body length and weight: no effects
- Other biological observations: no effects
- Bound residues forming a plateau: none
- Mortality and/or behavioural abnormalities of control: none
- Loss of test substance during test period: none
- Results with vehicle control: no effects - Validity criteria fulfilled:
- yes
- Conclusions:
- The test material showed steady state BCF values of 145 and 180 (mean 163) at test concentrations of 0.05 and 0.005 mg/L.
- Executive summary:
The study was performed according to the OECD Guideline for Testing of Chemicals 305 (Bioconcentration: Flow-through Fish Test) adopted on June 14, 1996 using two test material concentrations for exposure to Cyprinus carpio. The purpose of this study was to determine the bioconcentration potential of the test material in fish under flow-through conditions. Two environmental concentrations of the test material of 0.05 and 0.005 mg/L were used in this study. The exposure concentration was maintained using Tween 80 as solubiliser at 98 and 100 % of the nominal concentrations. The concentrations have been verified analytically using a HPLC method with UV detection. No signs of toxicity were observed throughout the study. The analysis revealed steady state BCFs of 145 and 180. Due to the low bioaccumulation potential, no depuration phase was required. The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 145 and 180 (mean 163).
Reference
Description of key information
The test item showed no relevant bioaccumulation. The steady state BCF values were at 145 and 180 (mean 163).
Key value for chemical safety assessment
- BCF (aquatic species):
- 163 dimensionless
Additional information
The study was performed according to the OECD Guideline for Testing of Chemicals 305 (Bioconcentration: Flow-through Fish Test) adopted on June 14, 1996 using two test material concentrations for exposure to Cyprinus carpio. The purpose of this study was to determine the bioconcentration potential of the test material in fish under flow-through conditions. Two environmental concentrations of the test material of 0.05 and 0.005 mg/L were used in this study. The exposure concentration was maintained using Tween 80 as solubiliser at 98 and 100 % of the nominal concentrations. The concentrations have been verified analytically using a HPLC method with UV detection. No signs of toxicity were observed throughout the study. The analysis revealed steady state BCFs of 145 and 180. Due to the low bioaccumulation potential, no depuration phase was required. The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 145 and 180 (mean 163) (reference 5.3.1-1).
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