sodium 3-[(3,4-dicyanophenyl)thio]propane-1-sulfonate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study test phase 2013-08-13 to 2013-08-15

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Report on substance meets the criteria for classification as reliable without restriction according to Klimisch et al (1997).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

lithium 3-((3,4-dicyanophenyl)thio)propane-1-sulfonate

EC Number:

700-787-7

Cas Number:

769953-18-6

Molecular formula:

C11H9N2O3S2Li

IUPAC Name:

lithium 3-((3,4-dicyanophenyl)thio)propane-1-sulfonate

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

At the start of the test the excess test solutions were sampled and at the end of the test one replicate of the control and 120 mg/L test solution was sampled.

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
A primary stock solution of test material with a nominal concentration of 120 mg/L was prepared by adding a nominal 120 mg of test substance to 1 litre of dilution water. The solution was then stirred for 5 minutes. The resultant stock was observed to be a clear and colourless solution and was used directly as the 120 mg/L test solution. All test solutions were clear and colourless.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
The test organism was the freshwater crustacean, Daphnia magna, obtained from continuous laboratory cultures held at Brixham Environmental Laboratory.

The stock cultures of D. magna were maintained in a reconstituted water medium, the same as the test dilution water, at a temperature of 20 +/- 2°C. The cultures were maintained in 2 L glass vessels with a working volume of 1.6 L. A photoperiod of 16 hours light:8 hours dark, with 20 minute transition periods was provided.

The D. magna cultures were fed on a mixed algae diet of Chlorella vulgaris, strain CCAP 211/12 and P. subcapitata, strain CCAP 278/4. The D. magna cultures were fed daily ad libitum depending on age and density of the culture. Culture conditions were such that the D. magna reproduction was by diploid parthenogenesis.

D. magna <24 hours old, obtained from a single culture vessel, were used for testing. The parent animals were 22 +/- 1 days old and had been maintained with a twice weekly renewal of reconstituted water medium since birth. The test organisms and the culture from which they were obtained showed no evidence of disease before the test period.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

Hardness of dilution water (as CaCO3) 228 mg/L

Test temperature:

20.4 C

pH:

8.00-8.46

Dissolved oxygen:

8.56-9.38 mg/L or 94-103% of the air saturation value

Nominal and measured concentrations:

The nominal concentrations were used for the calculation and reporting of results.

Details on test conditions:

TEST SYSTEM
Glass beakers of 250 mL nominal capacity were used as test vessels, with four replicates per test concentration. Each vessel contained 200 mL of test solution providing a depth of approximately 60 mm. The beakers were covered with loose fitting glass lids. The positions of the treatments were randomly allocated within the test area.

The test was initiated by the addition of 5 impartially selected D. magna, in <2.0 mL of dilution water, to each test vessel. Each treatment contained a total of 20 D. magna.

The nominal test solution temperature was 20 +/- 1°C, and a photoperiod of 16 hours light: 8 hours dark, with 20 minute dusk and dawn transition periods, was provided.

The test solutions were not aerated and the D. magna were not fed during the course of the study.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The reconstituted water medium used for testing (and maintenance of stock cultures) was Elendt's M4 D. magna medium.
- Total organic carbon: <1 mg/L
-Suspended solids at 105 C: <2.00 mg/L
- Pesticides:Highest organophosphorous pesticides <0.01ug/L, Highest organochlorine pesticides <0.01ug/L
- Alkalinity as CaCO3: 39.0 mg/L
- Conductivity: 620 uS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: none

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 hours light:8 hours dark, with 20 minute transition period.

EFFECT PARAMETERS MEASURED: An assessment of the response of the D. magna was made 24 and 48 hours after the commencement of the test. Each D. magna was viewed by eye and was defined as affected if showing no whole body movement, relative to the water, within a period of 15 seconds even if movement of individual appendages was visible. D. magna so affected were termed immobile.

TEST CONCENTRATIONS
- Test concentrations: 120 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: 0/20

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

It is concluded that as there were no significant mortalities or immobilisations in this study after 48h, and no other symptoms of toxicity were observed, that the test material has no toxic effect on Daphnia magna at 120 mg/L.

Executive summary:

Introduction

This study was undertaken to assess the acute toxicity of the test material to Daphnia magna. The study was designed to be compatible with the following guidelines:

OECD Guidelines for the Testing of Chemicals. Test Guideline 202, D.magna sp., Acute Immobilisation Test. Adopted 13 April 2004.

Results & Conclusions

In this study both the 24 and 48 hour EC50 were determined to be >120 mg/L. It is concluded that as there were no significant mortalities or immobilisations in this study after 48h, and no other symptoms of toxicity were observed, that the test material has no toxic effect on Daphnia magna at 120 mg/L.