4-(4-isopropoxyphenylsulfonyl)phenol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 1990-04-06 to 1990-05-31

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

The aliquot of the test substance was individually weighed and dissolved in 1 mL of acetone (including 0.01 mL of Tween 80) for each concentration. Each acetone solution was mixed with dilution water to prepare 5 L of the test solution and put into each glass vessel. The concentrations of the test solutions were 100; 56.2; 31.6; 17.8 and 10 mg/L.

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Red Killifish
- Strain: Oryzias latipes Schlegel 1850
- Source: Tropical Fish Center; Fujisawa, Kanagawa; Japan
- Age at study initiation: holding 29 days prior to test
- Length at study initiation: 2.8 ± 0.1 cm
- Weight at study initiation: 0.35 ± 0.07 g
- Feeding during test: none

ACCLIMATION
- Acclimation period: 29 days
- Type and amount of food: not specified
- Feeding frequency: once daily
- Health during acclimation (any mortality observed): 1 death during first 12 days; 1 death during the following 17 days

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Fish were observed for toxic sign or death at 3 and 6 hours after dosing and thereafter at 24-hour intervals for 96 hours. Fish were considered died when gill movement stopped and outer stimuli produced no reaction. Dead fish were removed when observed.

Hardness:

about 80 mg/L

Test temperature:

22.5 - 22.9 °C

pH:

7.7 - 8.1

Dissolved oxygen:

7.7 - 8.2 mg/L

Nominal and measured concentrations:

Nominal concentrations: 100; 56.2, 31.6; 17.8 and 10 mg/L

Details on test conditions:

Healthy 70 fish were selected, randomly assigned to each glass vessel and exposed for 96 hours. 5 exposed groups, a vehicle control and an untreated control group were used. The volume of the test water was 5 L per vessel; number of fish in each group 10 per vessel. All the test solutions including vehicle and untreated controls were renewed every 24 hours. Aeration was performed to maintain the dissolved oxygen content (> 60 %). Lighting was 16 hours (6:00 a.m. to 10:00 p.m. about 1000 lux on surface).

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

23.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95 % CI: 20.2 - 27.8 mg/L

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

22.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95 % CI: 18.5 - 27.0 mg/L

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

18.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95 % CI: 16.2 - 21.9 mg/L

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

18.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95 % CI: 16.2 - 21.9 mg/L

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Mortalities and toxic signs observed in each group are summarized in the table. Toxic signs (abnormal swimming) and deaths were observed in the 17.8 mg/L and higher dosed groups after 3 - 72 hours. Neither toxic signs nor deaths were found in the 10 mg/L and both control groups.

Reported statistics and error estimates:

The LC50 values at 24, 48, 72 and 96 hours were determined by Van der Waerden method.

Sublethal observations / clinical signs:

Table 1: Mortalities and Number of Dead Fish Exposed to D-8

Chemical	Concen-tration (mg/L)	No. of fish	No. of deaths (cumulative)^1)						Mortality (%) after 48 hours	Mortality (%) after 96 hours
			3	6	24	48	72	96
D-8	100	10	0 (2)	5 (3)	10	10	10	10	100	100
	56.2	10	0 (2)	5 (2)	10	10	10	10	100	100
	31.6	10	0 (3)	4 (1)	9	9	10	10	90	100
	17.8	10	0	0 (2)	1 (1)	2	2	2	20	20
	10.0	10	0	0	0	0	0	0	0	0
Vehicle control^2)	-	10	0	0	0	0	0	0	0	0
Untreated control	-	10	0	0	0	0	0	0	0	0

 

1): Figures in the parentheses indicates the number of fish showing abnormal swimming.

2): Acetone = 1 mL/5 L, Tween 80 = 0.01 mL/5 L

Validity criteria fulfilled:

yes

Conclusions:

D-8 was tested for acute toxicity to fish in a semistatic 96-hour test according to EU Method C.1. The LC50 value (96 hours) for D-8 in the Red Killifish was determined to be 18.8 mg/L.

Executive summary:

D-8 was tested for acute toxicity to fish in a 96-h semi-static test with five test and two control groups in accordance with EU Method C.1. The nominal test substance concentrations were 100; 56.2; 31.6; 17.8 and 10 mg/L. No analytical determination of the test item was performed. Based on the results from other aquatic studies where no renewal was performed and the concentrations on the solutions were analyticaly determined, it is concluded that the test item is stable in aquatic media and the studie is considered to be reliable. Deaths and toxic sign (abnormal swimming) were observed in the 17.8 mg/L group and higher exposed groups. Neither toxic signs nor deaths were found in the 10 mg/L group and both control groups.The EC50 value for 96 h exposure was found to be 18.8 mg/L and the NOEC was determined to be 10 mg/L.

Description of key information

D-8 was tested for acute toxicity to fish in a semistatic 96-hour test according to EU Method C.1. The LC50 value (96 hours) for D-8 in the Red Killifish was determined to be 18.8 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

18.8 mg/L

Additional information

D-8 was tested for acute toxicity to fish in a 96-h semi-static test with five test and two control groups in accordance with EU Method C.1. The nominal test substance concentrations were 100; 56.2; 31.6; 17.8 and 10 mg/L. No analytical determination of the test item was performed. Based on the results from other aquatic studies where no renewal was performed and the concentrations on the solutions were analyticaly determined, it is concluded that the test item is stable in aquatic media and the studie is considered to be reliable. Deaths and toxic sign (abnormal swimming) were observed in the 17.8 mg/L group and higher exposed groups. Neither toxic signs nor deaths were found in the 10 mg/L group and both control groups.The EC50 value for 96 h exposure was found to be 18.8 mg/L and the NOEC was determined to be 10 mg/L.