potassium;1-(4-chlorophenyl)pyrazol-3-olate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Deviations:

yes

Remarks:

starting dose was 200 mg/kg

Principles of method if other than guideline:

References for test guidelines given in the study report:
EEC Directive 92/69, Publication No. L383A, B.1: Acute Toxicity-oral, December 29, 1992;
Acute toxic class method, A national validation study of the ATC Method - an alternative to the LD50 test, Arch. Toxicol. 66: 455-470, 1992

GLP compliance:

yes (incl. QA statement)

Test type:

acute toxic class method

Test material

Test material information

Constituent 1

Reference substance name:

1-(4-chlorophenyl)pyrazol-3-ol

EC Number:

616-307-3

Cas Number:

76205-19-1

Molecular formula:

C9 H7 Cl N2 O

IUPAC Name:

1-(4-chlorophenyl)pyrazol-3-ol

Specific details on test material used for the study:

- Name of test material (as cited in study report): pyrazolon
- Physical state: crystalline powder, light-beige
- Analytical purity/Content: 100.5 g/ 100 g (H-NMR and IR spectroscopy)
- Lot/batch No.: 27967/95
- Storage condition of test material: room temperature, exclusion of light

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Dr. K. Thomae GmbH, Biberach, Federal Republic of Germany
- Age at study initiation: young adults
- Weight at study initiation: animals of comparable weight; 150-300g (+/- 20% of the mean weight)
- Fasting period before study: at least 16 hours before administration
- Housing: single housing, stainless steel wire mesh cages
- Diet (e.g. ad libitum): ad libitum (Kliba labor diet 343, assayed for chemical and microbiological contaminants)
- Water (e.g. ad libitum): ad libitum (tap water, regularly assayed for chemical contaminants)
- Acclimation period: at least 1 week


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 24 °C (no deviations from these ranges which influenced the results of the study)
- Humidity (%): 30 - 70% (no deviations from these ranges which influenced the results of the study)
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

CMC (carboxymethyl cellulose)

Details on oral exposure:

VEHICLE
(cleaned natriumcarboxymethylcellulose in aqua bidest.)
- Concentration in vehicle: 2 g/100 ml and 20 g/100 ml
- Amount of vehicle (if gavage): 10 ml/kg

CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: based on the physical and chemical characteristics of the test substance

Doses:

200 and 2000 mg/kg bw

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing:
clinical signs and symptoms: several times on the day of administration, at least once each workday;
mortality: twice each workday and once on Saturdays and Sundays;
body weights: d0 (shortly before application), weekly thereafter and at the end of the study (before fasting period)
- Necropsy of survivors performed: yes (withdrawl of food at least 16 hours before killing with CO2)

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

No mortalities occured

Clinical signs:

other: 200 mg/kg: no symptoms observed in males and females 2000 mg/kg: all males showed an impaired or poor general state, dyspnoea, staggering and piloerection, 2/3 males showed apathy; in female animals no abnormalities were observed

Gross pathology:

No findings