potassium;1-(4-chlorophenyl)pyrazol-3-olate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-(4-chlorophenyl)pyrazol-3-ol

EC Number:

616-307-3

Cas Number:

76205-19-1

Molecular formula:

C9 H7 Cl N2 O

IUPAC Name:

1-(4-chlorophenyl)pyrazol-3-ol

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was stirred in M4 medium at 20 °C for ca. 20 h. Undissolved test substance was removed by centrifugation (20 min at 17700 g)
- Eluate: The nominal concentration of the eluate was 100 mg/l

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2-24 h
- Feeding during test. none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.42 mmol/l

Test temperature:

20.2 - 20.6 °C

pH:

7.7 - 8.0

Dissolved oxygen:

7.8 - 8.4 mg/l

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
1.56,
3.13,
6.25,
12.5,
25,
50,
100,
control

Analytical test concentrations (mg/l) for nominal 3.13, 25 and 100 mg/l:
1.1,
8.8,
34.7

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom test tubes (20 ml)
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium according to ISO 10706
- Ca/mg ratio: 4:1
- Conductivity: 613 µS/cm
- Intervals of water quality measurement: after 0 and 48 h


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 day-night regime
- Light intensity: Artificial light, type warm white ( e.g. OSRAM L 58 W31), ca. 2 -7 µE(m²-s) at a wave length of 400 - 700 nm


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 3, 6, 24 and 48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

2.22 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

7.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

35.5 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Recovery rate of the test substance < 80 %

Reported statistics and error estimates:

Probit analysis