potassium;1-(4-chlorophenyl)pyrazol-3-olate

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-(4-chlorophenyl)pyrazol-3-ol

EC Number:

616-307-3

Cas Number:

76205-19-1

Molecular formula:

C9 H7 Cl N2 O

IUPAC Name:

1-(4-chlorophenyl)pyrazol-3-ol

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was directly added to the test medium

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Activated sludge from laboratory waste water treatment plants treating municipal and synthetic sewage
- Initial biomass concentration: 1 g/l

Study design

Total exposure duration:

30 min

Test conditions

Test temperature:

20 +/- 2 °C

pH:

6.5 - 6-7 (Before correction)
7.1 - 7.5 (After correction)

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
20,
100,
250,
500,
1000,
control

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer vessels (250 ml)
- Fill volume: 250 ml
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic medium (8 ml/vessel, 100-fold concentrated OECD medium)

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenole

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

45 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

240 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

30 min

Dose descriptor:

EC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate