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EC number: 205-758-8 | CAS number: 150-38-9
Toxicity to terrestrial plants
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to terrestrial plants: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- no data available
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: acceptable, well documented study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 227 (Terrestrial Plant Test: Vegetative Vigour Test)
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Details on sampling:
- no data available
- Vehicle:
- not specified
- Details on preparation and application of test substrate:
- A silty–loamy sand agricultural soil, USDA (Soil Survey Manual), was collected from 0 to 30 cm surface layer of the Melaten field in Aachen, Germany. The soil was air-dried at room temperature, sieved through a 2-mm sieve and characterised as follows:
pH: 6.8
organic matter: 3.5 %
soil fractions
sand: 49.4 %
Clay: 42.1 %
Silt: 8.5 % - Species:
- other: Nicotiana tabacum
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- Tobacco plants (N. tabacum SR-1) were used for the experiment.
- Test type:
- vegetative vigour test
- Study type:
- laboratory study
- Substrate type:
- natural soil
- Limit test:
- no
- Total exposure duration:
- 21 d
- Remarks:
- approximately 3 weeks
- Post exposure observation period:
- no data available
- Test temperature:
- 25/20 °C light/dark temperature regime
- pH:
- 6.40 - 6.83
- Moisture:
- 60% relative humidity
- Details on test conditions:
- 16 h light period (light intensity of 320 µmol/m2×s)
- Nominal and measured concentrations:
- nominal: 0.125,0.25, 1.25, 62.5, 125, and 250 mmol/kg for Na2EDTA
- Reference substance (positive control):
- not specified
- Species:
- other: Nicotiana tabacum
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 0.25 - < 1.25 other: mmol/kg
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Details on results:
- EDTA did not show any adverse effects at a concentration of < 0.125 mmol/kg. At 1.25 mmol/kg of EDTA the leaves showed necrotic lesions and the dry weight was negligible. At a concentration of 0.25 mmol/kg EDTA the dry weight is only slightly lower but the standard deviation is higher in comparison to the 0.125 mmol/kg application.
- Results with reference substance (positive control):
- no data available
- Reported statistics and error estimates:
- no data availble
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on the results of this vegetative vigour study EDTA had an inhibitory effect on growth of Nicotiana tabacum at rate of 1.25 mmol/kg soil (equivalent to 420 mg/kg). The NOEC will be determined to be 0.25 mmol/kg soil (equivalent to 84 mg/kg soil).
- Executive summary:
The effects of EDTA (Na2EDTA) on vegetative vigour of plants following application were examined in a laboratory test on Nicotiana tabacum. The test item was applied at a dose rate of up to 250 mmol/kg and compared to an untreated control.
According to the results, the test item had an inhibitory effect on growth of the tested species at rates of 1.25 mmol/kg soil (equivalent to 420 mg/kg) and thus determined to be the overall LOEC for this species.
The overall NOEC was determined to be 0.25 mmol/kg soil (equivalent to 84 mg/kg).
- Endpoint:
- toxicity to terrestrial plants: long-term
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Refer to the Analogue Approach Justification document provided in Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Species:
- other: Nicotiana tabacum
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 0.25 - < 1.25 other: mmol/kg
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks on result:
- other: equivalent to 84 mg/kg soil
- Endpoint:
- toxicity to terrestrial plants: long-term
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- no data available
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: acceptable, well documented study
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- seedlings of Phaseolus vulgaris were exposed to EDTA for 14 days
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Species:
- Phaseolus vulgaris
- Plant group:
- Dicotyledonae (dicots)
- Test type:
- seedling emergence toxicity test
- Study type:
- laboratory study
- Substrate type:
- other: seedlings grown in polystyrene squares
- Limit test:
- no
- Total exposure duration:
- 14 d
- Species:
- Phaseolus vulgaris
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 58.4 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Validity criteria fulfilled:
- not specified
- Conclusions:
- In a 14 day eposure test EDTA showed no inhibitory effect on seedling emergence and growth of Phaseolus vulgaris up to a concentration of 58.4 mg/kg soil.
- Executive summary:
In a 14 day eposure test EDTA showed no inhibitory effect on seedling emergence and growth of Phaseolus vulgaris up to a concentration of 58.4 mg/kg soil.
Referenceopen allclose all
Description of key information
NOEC (21 d): 84 mg/kg soil
Key value for chemical safety assessment
Additional information
Since no studies investigating the toxicity trisodium hydrogen EDTA (CAS 150-38-9) to terrestrial plants are available, in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5 a read-across to a structurally related EDTA species was conducted. This read-across is justified in detail in the overall summary (IUCLID Section 6.3) and within the analogue justification in IUCLID Section 13.
According to Evangelou et al. (2006) disodium dihydrogen EDTA (CAS 139-33-3) showed adverse effects on growth of Nicotiana tabacum. The study was performed according to OECD 227. Seedlings of tobacco plants were exposed for 21 d in natural soil and the dry weight was analysed. Even though no NOEC was derived, the dry weight of plants strongly decreased at a concentration of 1.25 mmol/kg. Thus, the next lower concentration can be considered as NOEC value (0.25 mmol/kg equivalent to 84 mg/kg soil).
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