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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2019

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Isopropyl nitrite
EC Number:
208-779-0
EC Name:
Isopropyl nitrite
Cas Number:
541-42-4
Molecular formula:
C3H7NO2
IUPAC Name:
propan-2-yl nitrite

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Remarks:
acetone

Test organisms

Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:
not specified
Water media type:
other: ultrapure water
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
23 +/- 2°C
pH:
7.8
Nominal and measured concentrations:
Nominal: 0; 0.95; 3.05; 9.77; 31.25 and 100 mg/L
Due to the instability of test item, the calculations were performed on the nominal concentrations.
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
15.79 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
4.85 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: yield

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 of Isopropyl nitrite after a 72-hour exposure period was calculated to be 15.79 mg/L (95% confidence limits: 13.31 – 18.4 mg/L), based on growth rate, and 4.85 mg/L (95% confidence limits: 3.48 – 6.61 mg/L).