Isopropyl nitrite

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Report date:

2019

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Isopropyl nitrite

EC Number:

208-779-0

EC Name:

Isopropyl nitrite

Cas Number:

541-42-4

Molecular formula:

C3H7NO2

IUPAC Name:

propan-2-yl nitrite

Sampling and analysis

Analytical monitoring:

yes

Remarks:

GC-MS/MS

Test solutions

Vehicle:

yes

Remarks:

acetone

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

other: deionised water

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

Between 140 and 250 mg/L

Test temperature:

20 °C ± 2

pH:

between 6 and 9

Dissolved oxygen:

7.9 mg/L

Nominal and measured concentrations:

Nominal concentrations: 0; 0.95; 1.7; 3.1; 5.6 and 10 mg/L.
Due to the instability of test item, the calculations were performed on the nominal concentrations.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.123 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

The EC50 with reference item over a period of 24 hours was between 0.6 and 2.1 mg.L-1 (actual value: 1.129 mg.L-1).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on the number of immobilized daphnids, the EC50 after a 24-hour exposure period was calculated to be 2.968 mg.L-1 (2.876 – 3.060 mg.L-1 95% confidence limits) and the EC50 after a 48-hour exposure period was calculated to be 2.123 mg.L-1 (1.915 – 2.331 mg.L-1 95% confidence limits).