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EC number: 202-114-8
CAS number: 91-99-6
Table 1: Biodegradation Percentages at Different Points in Time
% Biodegradation after x days(1)
Procedure control A(2)
Procedure control B(2)
Procedure control mean A and B
Difference between duplicate values
Test item low A(3)
Test item low B(3)
Test item low mean A and B
Test item high A(3)
Test item high B(3)
Test item high mean A and B
Toxicity control A
2 + 1(4)
Toxicity control B
Toxicity control mean A and B
(1): Except for the percentages biodegradation, all calculations were performed without rounding off.
(2): ThOD positive control sodium acetate: 0.78 mg O2/mg
(3): ThOD test item: 2.54 mg O2/mg
(4): Toxicity control contains sodium acetate and test item.
ready biodegradation of the substance under the conditions of the closed
bottle test was investigated according to OECD guideline 301 D and GLP
principles. Test concentrations of 1 and 3 mg/L were tested in duplicate
during 28 days. The relative biodegradation values calculated from the
O2 measurements performed during the test period of 28 days revealed no
significant biodegradation of the substance. Since biodegradation of at
least 60% was not reached within 14 days of biodegradation exceeding
10%, the substance was concluded to be not readily biodegradable. In the
toxicity control, the substance was found not to inhibit microbial
activity. The criteria for acceptability of the test were met and the
study is considered to be reliable without restriction. In conclusion,
the substance was determined to be not readily biodegradable under the
conditions of the closed bottle test.
substance was determined to be not readily biodegradable under the
conditions of the closed bottle test (OECD 301D). The
substance was found not to inhibit microbial activity.
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