Reaction mass of sulfonium, dodecylethyl[1-(2-methoxy-2-oxoethyl)-3-oxo-3-(pentyloxy)propyl]-, tetrafluoroborate(1-)(1:1) and sulfonium, dodecylethyl[3-methoxy-1-(2-methoxy-2-oxoethyl)-3-oxopropyl]-, tetrafluoroborate(1-)(1:1) and sulfonium, dodecylethyl[3-oxo-1-[2-oxo-2-(pentyloxy)ethyl]-3-(pentyloxy)propyl]-, tetrafluoroborate(1-)(1:1)

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Deviations:

no

Remarks:

No deviations occurred that impacted the integrity of the study.

GLP compliance:

yes

Test type:

acute toxic class method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

(1,5-dimethoxy-1,5-dioxopentan-3-yl)(dodecyl)ethylsulfanium; [1,5-dioxo-1,5-bis(pentyloxy)pentan-3-yl](dodecyl)ethylsulfanium; dodecyl(ethyl)[1-methoxy-1,5-dioxo-5-(pentyloxy)pentan-3-yl]sulfanium; tris(tetrafluoroboranuide)

EC Number:

943-993-4

Cas Number:

2220260-54-6

Molecular formula:

not applicable for multi-constituent.

IUPAC Name:

(1,5-dimethoxy-1,5-dioxopentan-3-yl)(dodecyl)ethylsulfanium; [1,5-dioxo-1,5-bis(pentyloxy)pentan-3-yl](dodecyl)ethylsulfanium; dodecyl(ethyl)[1-methoxy-1,5-dioxo-5-(pentyloxy)pentan-3-yl]sulfanium; tris(tetrafluoroboranuide)

Test material form:

liquid

Details on test material:

Multi-constituent substance.
degree of purity: >=94%

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Batch 624730
- Expiration date of the lot/batch: 2017-12
- Purity test date: 05 May, 2017

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Stability under test conditions: No data
- Solubility and stability of the test substance in the solvent/vehicle: Dosed neat

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Dosed neat

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River, Raleigh NC and Stone Ridge NY
- Females nulliparous and non-pregnant: Yes
- Age at study initiation: 7-8 weeks
- Weight at study initiation: 181-208 grams
- Fasting period before study: 16-20 hours prior to dosing.
- Housing: Animals were housed in suspended wire cages; five per sex per cage prior to dosing and three per sex per cage following dosing.
- Diet (e.g. ad libitum): Fresh PMI Rat Chow (Diet No. 5012), ad libitum
- Water (e.g. ad libitum): Water, ad libitum
- Acclimation period: At least 5 days.

ENVIRONMENTAL CONDITIONS
- Temperature (°C): No data
- Humidity (%): No data
- Air changes (per hr): No data
- Photoperiod (hrs dark / hrs light): 12/12

IN-LIFE DATES: From: 11 July 2017 To: 09 August, 2017

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

VEHICLE: None

MAXIMUM DOSE VOLUME APPLIED: 20 mL/kg

DOSAGE PREPARATION: Neat

CLASS METHOD
- Rationale for the selection of the starting dose: Based on information from the sponsor.

Doses:

2000 mg/kg bodyweight

No. of animals per sex per dose:

6 females

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Animals were observed at 15 minutes, 1, 2 and 4 hours post-dose and once daily for 14 days for toxicity and pharmacological effects and twice daily for mortality.Body weights were recorded at pretest, weekly and at termination.
- Necropsy of survivors performed: Yes, gross examination.
- Other examinations performed: clinical signs, body weight, gross pathology.

Results and discussion

Effect levels

Key result

Sex:

female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

act. ingr.

Mortality:

No mortality occurred during the study.

Clinical signs:

other: Abnormal physical signs including wetness of the nose/mouth area, soiling of the anogenital area and piloerection.

Gross pathology:

The gross necropsy revealed no observable abnormalities.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Based on the results of the study, the test article has an oral LD50 value greater than 2,000 mg/kg body weight in female rats.

Executive summary:

The acute oral lethality potential of MTDID 47403 was evaluated in female Sprague Dawley rats. The study was conducted according to OECD 423 under OECD GLP conditions. Initially, three rats were dosed via oral gavage with unchanged test article at 2000 mg/kg bodyweight. In addition, three more females were dosed as a confirmatory group at 2000 mg/kg. The rats were observed at 15 minutes, 1, 2, and 4 hours post-dose and once daily for 14 days for toxicity and pharmacological effects, and twice daily for mortality. Body weights were recorded immediately pretest, weekly and at termination. All animals were examined for gross pathology. No mortality occurred during the study. Abnormal physical signs including wetness of the nose/mouth area, soiling of the anogenital area and piloerection. All six animals gained body weight by study termination and no abnormal findings were observed upon gross necropsy. Based on the results of the study, the test article has an oral LD50 value greater than 2,000 mg/kg body weight in female rats.