Registration Dossier

Administrative data

Endpoint:
eye irritation: in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report Date:
2017

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 437 (Bovine Corneal Opacity and Permeability Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Details on test material:
Multi-constituent substance.
degree of purity: >=94%
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Batch 624730
- Expiration date of the lot/batch: 2017-12
- Purity test date: 05 May, 2017

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Stability under test conditions: No data
- Solubility and stability of the test substance in the solvent/vehicle: Dosed neat

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Dosed neat

Test animals / tissue source

Species:
cattle
Details on test animals or tissues and environmental conditions:
SOURCE OF COLLECTED EYES
- Source: Spear Products
- Number of animals: No data
- Characteristics of donor animals (e.g. age, sex, weight): At least six months old
- Storage, temperature and transport conditions of ocular tissue (e.g. transport time, transport media and temperature, and other conditions): Eyes were transported to the laboratory in a solution containing Hanks' Balanced Salt Solution (HBSS) and penicillin-streptomycin in a refrigerated container.
- Time interval prior to initiating testing: No data
- indication of any existing defects or lesions in ocular tissue samples: All eyes were examined prior to use on the day of dosing. Any eye with a cornea exhibiting evidence of vascularization, pigmentation, opacity or scratches was discarded.
- Indication of any antibiotics used: Penicillin-streptomycin.

Test system

Vehicle:
unchanged (no vehicle)
Controls:
yes, concurrent positive control
yes, concurrent negative control
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.75 mL

VEHICLE: None
Duration of treatment / exposure:
10 minutes
Number of animals or in vitro replicates:
3 corneas/group
Details on study design:
SELECTION AND PREPARATION OF CORNEAS: The eyes were examined prior to use on the day of dosing. Any eye with a cornea exhibiting evidence of vascularization, pigmentation, opacity or scratches was discarded.

QUALITY CHECK OF THE ISOLATED CORNEAS: The eyes were examined prior to use on the day of dosing. Any eye with a cornea exhibiting evidence of vascularization, pigmentation, opacity or scratches was discarded.

NUMBER OF REPLICATES: 3 per treatment group.

NEGATIVE CONTROL USED: Minimal Essential Media

SOLVENT CONTROL USED: Not applicable

POSITIVE CONTROL USED: Ethanol

APPLICATION DOSE AND EXPOSURE TIME: 0.75 mL

TREATMENT METHOD: Open chamber

POST-INCUBATION PERIOD: Yes, 4 hours

REMOVAL OF TEST SUBSTANCE
- Number of washing steps after exposure period: At least two.
- POST-EXPOSURE INCUBATION: 4 hours

METHODS FOR MEASURED ENDPOINTS:
- Corneal opacity:
- Corneal permeability: passage of sodium fluorescein dye measured with the aid of [UV/VIS spectrophotometry / microtiter plate reader] (OD490)
- Others (e.g, pertinent visual observations, histopathology): (please specify)

SCORING SYSTEM: In Vitro Irritancy Score (IVIS)

DECISION CRITERIA: IVIS Score:
0-3: Non-irritant (Not Classified)
3.1-25: Mild Eye Irritant
25.1 to 55: Moderate Eye Irritant
55.1 and greater: Severe/Corrosive Eye Irritant

Results and discussion

In vitro

Results
Irritation parameter:
in vitro irritation score
Run / experiment:
Mean
Value:
3.03
Vehicle controls validity:
not applicable
Negative controls validity:
valid
Positive controls validity:
valid
Remarks on result:
no indication of irritation

In vivo

Irritant / corrosive response data:
Corrected Mean Optical Density: 0.002
Corrected Mean Opacity Score: 3.00
Mean OD (490 nm) (Permeability): 0.021

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
Based on the results of the study (IVIS=3.03), MTDID 47403 is considered non-irritating in the Bovine Corneal Opacity and Permeability Test (BCOP).
Executive summary:

The ocular irritation and corrosion potential of MTDID 47403 was evaluated in the Bovine Corneal Opacity Permeability (BCOP) test. The test method was based on OECD 437 and was performed in compliance with OECD GLP. The bovine eyes were examined prior to use on the day of dosing. Any eye with a cornea exhibiting evidence of vascularization, pigmentation, opacity or scratches was discarded. Corneas from eyes that were free of defects were dissected from the surrounding tissues. A 2-3 mm rim of sclera was left attached to each cornea. The corneas were then placed in a container of fresh HBSS. The dissected corneas were mounted in holders and placed in chambers filled with MEM solution. The entire holder was incubated at 32°C for at least 1 hour but no longer than 2. Corneas (3/group) were treated with 0.75 mL of MTDID 47403 for 10 minutes at 32°C. A positive control (Ethanol) and a negative control (MEM solution), were tested in parallel with the test material. At the end of the exposure period, the corneas were rinsed with a MEM solution containing phenol red; a final rinse was made with MEM without phenol red. Opacity was evaluated using the OP-KIT following the 10 minute exposure. Following opacity readings, the cell culture medium was replaced with 1.0 ml of 0.4% sodium fluorescein solution in Dulbecco's phosphate buffered saline (PBS) and incubated for approximately 90 minutes at 32°C. Following the 90-minute sodium fluorescein exposure, permeability was measured as the optical density at 490 nm by a spectrophotometer. The In Vitro Irritancy Score (IVIS) for the test material, vehicle control, and positive control was calculated by adding the corrected mean opacity score to fifteen times the corrected mean optical density; the IVIS for negative control was performed by adding the mean opacity score to fifteen times the mean optical density. Based on the IVIS score, the test article was classified according to the prediction model described in DB-ALM Protocol No. 127, a modification of the prediction model suggested by Gautheron, et al. (1994). The mean IVIS for the test article was 3.03. The 10 minute mean corrected opacity score was 3.00 and the corrected mean optical density score was 00.002. Controls performed as expected. Based on the results of the study (IVIS=3.03), MTDID 47403 is considered non-irritating in the Bovine Corneal Opacity and Permeability Test (BCOP).