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EC number: 214-333-6 | CAS number: 1121-60-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001-10-01 to 2001-10-05
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- (The Department of Health of the Government of the United Kingdom)
- Analytical monitoring:
- yes
- Details on sampling:
- The concentration and stability of the test material in the test solutions were verified by chemical analysis at 0, 24 and 96 hours .
- Concentrations: 1.0 , 1.8 , 3.2 , 5.6 and 10 mg/l
- Sample storage conditions before analysis: Duplicate samples and samples at 24 (fresh media), 48 and 72 hours (fresh and old media) were taken and stored frozen (approximately -20°C) for further analysis if necessary. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: An amount of test material (500 mg) was dissolved in dechlorinated tap water with the aid of ultrasonication for approximately 10 minute and the volume adjusted to 1 litre to give the 500 mg/l stock solution. Aliquots of this stock solution (40, 72, 128, 224 and 400 ml) were each separately dispensed into a final volume of 20 litres of dechlorinated tap water with the aid of a flat bladed mixer for approximately 1 minute to give the 1.0,1.8, 3.2, 5.6 and 10 mg/l test concentrations.
Prior to use the stock solution was inverted several times to ensure adequate mixing and homogeneity.
The Sponsor indicated that the test material might be light sensitive and therefore the test material was weighed under a non-actinic light source. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout
- Source: Brow Well Fisheries Limited, Hebden, near Skipton, Yorkshire, UK , maintained in-house since 14 September 2001.
- Age at study initiation : juvenile
- Length at study initiation (mean): 5.1 cm (sd = 0.2)
- Weight at study initiation (mean): 1.03 g (sd = 0.12)
- Feeding during test : none
ACCLIMATION
- Acclimation period: 19 September 2001 to 1 October 2001
- Acclimation conditions : same as test
- Type and amount of food: commercial trout pellets
- Health during acclimation : There was zero mortality in the 7 days prior to the start of the test - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- appr. 100 mg/l as CaCO3
- Test temperature:
- 14 °C , measured at test start and after each test media renewal at 24 , 48 and 72 hours .
- pH:
- 7.4 - 8.0 , measured at test start and after each test media renewal at 24 , 48 and 72 hours .
- Dissolved oxygen:
- 9.0 - 9.8 mg O2/l (87-95% of saturation) , measured at test start and after each test media renewal at 24 , 48 and 72 hours .
- Nominal and measured concentrations:
- see table 1(at any other information on materials incl. tables)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type : The test vessels were covered to reduce evaporation
- Material, size, headspace, fill volume: glass tanks , containing 20 l test solution
- Aeration : The test vessels were aerated via narrow bore glass tubes.
- Renewal rate of test solution : daily
- No. of organisms per vessel : 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.52 g bw/l
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Laboratory tap water was dechlorinated by passage through an activated carbon filter (Purite Series 500) and partly softened (Elga Nimbus 1248D Duplex Water Softener) . After dechlorination and softening the water was passed through a series of computer controlled plate heat exchangers to achieve the required temperature.
OTHER TEST CONDITIONS
- Light intensity : The test vessels were shielded from the light as information provided by the Sponsor indicated the test material might be of a light sensitive nature.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Range finding study
- Test concentrations : 1.0 , 10 and 100 mg/l
- Results used to determine the conditions for the definitive study : The results showed no mortalities at the test concentration of 1.0 mg/l. However, mortalities were observed at 10 and 100 mg/l . Based on this information test concentrations of 1.0, 1.8, 3.2, 5.6 and 10 mg/l were selected for the definitive study. - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% confidence limit : 1.0 - 1.8 mg/l
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Mortality data :
Cumulative mortality data from the exposure of rainbow trout to the test material during the definitive study are given in Table 2 .
Sub-lethal effects :
Sub-lethal effects of exposure were observed at test concentrations of 1.8 mg/l and above. These responses were increased pigmentation and swimming at the bottom of the test vessels with increased pigmentation (see Table 3). - Validity criteria fulfilled:
- yes
- Remarks:
- The study was performed in compliance with UK GLP standards (Schedule 1, Good Laboratory Practice Regulations 1999 (SI 1999/3106)). These Regulations are in accordance with GLP standards published as OECD Principles on Good Laboratory Practice
- Conclusions:
- The study is regarded as a valid guideline study with certificated GLP compliance. The acute toxicity of the test material to the freshwater fish rainbow trout (Oncorhynchus mykiss) has been investigated and resulted in a 96-h LC50 value of 1.3 mg/L with 95% confidence limits of 1.0-1.8 mg/L. The NOEC was determined with 1.0 mg/L.
- Executive summary:
The acute toxicity to fish of Pyridine-2-aldehyde was tested according to OECD guideline 203 and EU Method C.1 in a semi-static freshwater test with rainbow trout (Oncorhynchus mykiss) as test organisms, which is a freshwater fish representative of a wide variety of natural habitats, and can therefore be considered as an important non-target organism in freshwater ecosystems. The study was conducted under certificated GLP compliance. Following a preliminary range-finding study, fish were exposed, in groups of ten, to an aqueous solution of the test material over a range of concentrations of 1.0, 1.8, 3.2, 5.6 and 10 mg/L for a period of 96 hours under semi-static test conditions. The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the study until termination after 96 hours.
The 96 -h LC50 based on nominal test concentrations was 1.3 mg/L with 95% confidence limits of 1.0 -1.8 mg/L. The NOEC was determined with 1.0 mg/L.
Analysis of the test solutions at 0, 24 and 96 hours showed measured test concentrations to range from 84% to 112% of nominal values and therefore the results are based on nominal test concentrations only.
Reference
Description of key information
A 96 -h LC50 value of 1.3 mg/L to the freshwater fish rainbow trout (Oncorhynchus mykiss) was determined for the substance under the conditions of the test. It was determined in a reliable study conducted according to an appropriate standard tes method (OECD 203).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1.3 mg/L
Additional information
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