1-[4-(2-methylpropyl)phenyl]ethan-1-one

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))

Deviations:

yes

Remarks:

The oxygen content measurement time was higher than 10 minutes (i.e. 10.1 - 10.5 min). An influence to the results of this study can be excluded.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1-[4-(2-methylpropyl)phenyl]ethan-1-one

EC Number:

254-159-8

EC Name:

1-[4-(2-methylpropyl)phenyl]ethan-1-one

Cas Number:

38861-78-8

Molecular formula:

C12H16O

IUPAC Name:

1-[4-(2-methylpropyl)phenyl]ethan-1-one

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Application of the test substance: Direct addition by initial weight into the test flasks. The test substance was weighed in the required amounts for the test concentrations directly to the test vessels.

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

Origin of inoculum: aeration tank of the wastewater treatment plant of Mannheim, Germany

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

180 min

Test conditions

Test temperature:

19.9 - 20.7 °C

pH:

7.5

Dissolved oxygen:

> 2 mg/L during aeration

Nominal and measured concentrations:

Nominal: 1000, 500, 250, 125, 62.5 mg/L and control
Reference substamce: 100, 10 and 1 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glas beaker (nominal 1 L)
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 500 ml fill volume
- Aeration: yes
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per toxic control (replicates): 6
- Sludge concentration (weight of dry solids per volume): 1.5 g/L dry weight


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD 209
- Particulate matter: not applicable

OTHER TEST CONDITIONS
- Adjustment of pH: yes

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen consumption
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

40 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Effect concentrations 2

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

280 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Results with reference substance (positive control):

The EC50 of 3,5-dichlorophenol was 8.2 mg/L

Reported statistics and error estimates:

The consumption rates were used for the determination of the ECx by the probit method based on Finney with the software TOXRAT Professional 2.10. The effect concentrations were given with an accuracy of 2 significant digits.