Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report Date:
2018

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
Inoculum: Activated sludge from a municipal sewage plant
Origin: Municipal waste water treatment plant Mannheim, Germany
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
20 mg/L
Based on:
TOC
Initial conc.:
ca. 24 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Composition of medium: according to OECD 301B
- Additional substrate: no
- Solubilising agent (type and concentration if used): no
- Test temperature: 22 +/- 2 °C
- pH: 7.1 - 7.4
- pH adjusted: yes, to pH 7.4
- Aeration of dilution water: yes
- Continuous darkness: yes/no


TEST SYSTEM
- Culturing apparatus: brown glass bottles
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: aeration with CO2 free air
- Test performed in closed vessels due to significant volatility of test substance: no
- Test performed in open system: yes
- Details of trap for CO2 and volatile organics if used: KOH


SAMPLING
- Sampling frequency: variable
- Sterility check if applicable: no
- Sample storage before analysis: no


CONTROL AND BLANK SYSTEM
- Inoculum blank: 1
- Toxicity control: 1


STATISTICAL METHODS: not applicable
Reference substance
Reference substance:
aniline

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (CO2 evolution)
Value:
0 - 10
Sampling time:
12 d
Parameter:
% degradation (CO2 evolution)
Value:
10 - 20
Sampling time:
14 d
Parameter:
% degradation (CO2 evolution)
Value:
40 - 50
Sampling time:
24 d
Key result
Parameter:
% degradation (CO2 evolution)
Value:
50 - 60
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
day 2: 0 - 10 %
day 5: 30 - 40 %
day 8: 60 - 70 %
day 14: 80 - 90 %
day 28: 90 - 100 %

Applicant's summary and conclusion