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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: screening test, other
Remarks:
Test wa performed acc to DIN 38412 part 25, similar to OECD 302 B; no GLP
Type of information:
experimental study
Adequacy of study:
key study
Study period:
17 May 1989 - 14 June 1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Deviations:
yes
Remarks:
origins of the sludge and test substance concentration
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method C.9 (Biodegradation: Zahn-Wellens Test)
Deviations:
yes
Remarks:
already mentioned
Principles of method if other than guideline:
DIN 38 412 Part 25 (1984). This method is corresponding with small differences to the Guideline OECD 302B and EU Test method C.9.
GLP compliance:
no
Remarks:
newly generated from 1989 report
Specific details on test material used for the study:
Test substance dissolved in approx. 30% in aqueous solution
Oxygen conditions:
aerobic
Inoculum or test system:
sewage, industrial, non-adapted
Details on inoculum:
Mixture of activated sludge from the sewage treatment plant from one industrial site and activated sludge from the other industrial site.
Duration of test (contact time):
28 d
Initial conc.:
357.9 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
Stock solution:
5 g 30% acetoacetamide solution were mixed with 1800 ml de-ionized water, the pH adjusted from 6.9 to 7.1. This batch was stirred over a period of 24 hours at 22°C. The resulting clear solution was filled up with de-ionized water to a final volume of 2000 ml and used as stock solution.
Inoculum:
Washing the sediment from the activated sludge two times by centrifuging and resuspending in mineral medium. 12 g/L (=1240 mg dry mass/L) was used for the batch.12 g/L of wet centrifuged sludge was used as inoculum.
241 ml/L stock solution was added. Test item concentration: 357.9 mg/L

Reference substance:
diethylene glycol
Remarks:
271.6 mg DOC/L
Test performance:
During the first 7 days of the test the DOC exhaustion achieved >70% of the carbon content of the added test substance, but during the following 7 days the rate of degradation stayed at the plateau in the range of 70% to 72%.
Parameter:
% degradation (DOC removal)
Value:
32
Sampling time:
1 d
Parameter:
% degradation (DOC removal)
Value:
70
Sampling time:
9 d
Parameter:
% degradation (DOC removal)
Value:
68
Sampling time:
21 d
Key result
Parameter:
% degradation (DOC removal)
Value:
64
Sampling time:
28 d
Remarks on result:
other: inherently biodegradable
Details on results:
The results were confirmed by additionally conducted COD control analyses. The analysis of the COD before adding the inoculum resulted in a start value of 510 mg/L COD from the test substance in the test batch. The final COD (28 days, test minus blank) was 160 mg/L, corresponding to 69% elimination.
Validity criteria fulfilled:
yes
Interpretation of results:
inherently biodegradable
Conclusions:
Acetoacetamide (30% solution) is inherently biodegradable under the conditions of the zahn-Wellens Test.
Executive summary:

A 30% aequous solution of acetoacetamide was tested for inherent biodegradation in the Zahn-Wellens-test by measuring the DOC disappearance. During the first 7 days of the test the DOC exhaustion achieved > 70% of the carbon content of the added test substance, but the following day the rate of degradation staye at the plateau in the range 70 - 72%. The results were confirmed by additionally conducted COD control analyzes. COD at the start was 510 mg/L and 160 mg/L after 28 days, corresponding to an elimination of about 69%.

Acetoacetamide (30% solution) is inherently biodegradable under the conditions of the zahn-Wellens Test.

Description of key information

Acetoacetamide (30% solution) is inherently biodegradable under the conditions of the zahn-Wellens Test.

Key value for chemical safety assessment

Biodegradation in water:
inherently biodegradable
Type of water:
freshwater

Additional information

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