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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
22 March 1989 - 14 April 1989
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
not specified
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Product No.: HNAA 995
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
Dilution water was used according to ISO/DIS 734611.
Concentration: 500, 5000 and 10000 mg/L
pH between 8 and 8.2
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
- Common name: Brachydanio rerio
- Strain:Hamilton-Buchanan
- Source: West Aquarium, 3422 Bad Lauterberg
- Age at study initiation: about 3 months
- Length at study initiation: 2.5 - 3.5 cm average: 3 cm
- Feeding during test: twice a day ad libitum
- Food type: Tetra Min, Tetra Werke. Melle

ACCLIMATION
- Acclimation period: at least 1 days before start of the tests
- Acclimation conditions: same as test:
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
21.2 - 23.0 °C
pH:
7.7 - 8.7
Dissolved oxygen:
about 9 mg/L
Nominal and measured concentrations:
Nominal: 5000 mg/L measured (average) 4985.7 mg/L
Nominal: 10000 mg/L measured (average) 10098 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: open Glass bassin (30 x 22 x 24 cm), calibrated for 10 Liters, standing in a waterbath made from plastics.
The temperature of the water bath was controlled for 22+-1 °C by a thermostate
- Aeration: no aeration except the 1000 mg/L group after 48 hours

OTHER TEST CONDITIONS
- Photoperiod: day / night: 12 h / 12 h
- Light intensity: 700 Lux
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
500 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 0 from 30 fish
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
5 000 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 0 from 10 fish
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 5 000 - < 10 000 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: LC80
Effect conc.:
10 000 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 8 from 10 fish
Details on results:
- Behavioural abnormalities: increased breathing, increased or decreade activity, swim posture tail-heavy,
Integument dark colored
Validity criteria fulfilled:
yes
Conclusions:
For the 30% acetoacetamid solution the LC50 was determined as 5000 to 10000 mg/L and LC0=5000 mg/L.
This means, for the pure substance these values can be calculated as
LC0 = 1500 mg/L (pure substance) and
LC50 = 1500 - 3000 mg/L (pure substance)
Therefore, acetoacetamid is not subject for classification concerning the toxicity to fish.
Executive summary:

A 30 % solution of acetoacetamide was tested for toxicity to zebra fish acc. OECD 203 guideline. Nominal concentrations of 500 mg/L, 5000 ml/L and 10000 mg/L were used. After 96 hours no mortality was observed

up to 5000 mg/L. For 10000 mg/L after 48 hours 6 of 10 fish have died and after 96 hours 8 of 10 fish. For LC 50 only a range can be given as LC50 = 5000 to 10000 mg/L and for the LC0=5000 mg/L.

Calculated for the pure substance: LC50 = 1500 - 3000 mg/L. Therefore, acetoacetamid is not subject for classification concerning the toxicity to fish.

Description of key information

A 30 % solution of acetoacetamide was tested for toxicity to zebra fish acc. OECD 203 guideline. Nominal concentrations of 500 mg/L, 5000 ml/L and 10000 mg/L were used. After 96 hours no mortality was observed

up to 5000 mg/L. For 10000 mg/L after 48 hours 6 of 10 fish have died and after 96 hours 8 of 10 fish. For LC 50 only a range can be given as LC50 = 5000 to 10000 mg/L and for the LC0=5000 mg/L.

Calculated for the pure substance: LC50 = 1500 - 3000 mg/L.Therefore, acetoacetamid is not subject for classification concerning the toxicity to fish.

Key value for chemical safety assessment

Additional information

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