Registration Dossier

Administrative data

Endpoint:
partition coefficient
Type of information:
experimental study
Adequacy of study:
key study
Study period:
15 - 16 Nov 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report Date:
2018

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 117 (Partition Coefficient (n-octanol / water), HPLC Method)
Version / remarks:
April 13, 2004
Deviations:
no
GLP compliance:
yes
Type of method:
HPLC method
Partition coefficient type:
octanol-water

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder

Study design

Analytical method:
high-performance liquid chromatography

Results and discussion

Partition coefficient
Type:
log Pow
Partition coefficient:
>= 1.9 - <= 6.6
Temp.:
25 °C
Remarks on result:
other: pH not reported
Remarks:
17 peaks were identified, the single results are reported in "Any other information on results incl. tables)

Any other information on results incl. tables

Calibration curve

The calibration curve obtained using the reference items was:

log Pow = 2.250 * log k + 1.925

r = 0.996

Results

Peak no.

Test solution A

log Pow

(measured value)

Test solution A

Peak area

(%)

Test solution B

log Pow

(measured value)

Test solution B

Peak area

(%)

Average log Pow

1

1.9

1.92

1.9

1.90

1.9

2

2.1

1.54

2.1

1.57

2.1

3

3.1

1.85

3.1

1.78

3.1

4

3.3

1.10

3.3

1.09

3.3

5

3.8

1.48

3.8

1.47

3.8

6

4.0

2.25

4.0

2.19

4.0

7

4.2

1.85

4.2

1.83

4.2

8

4.3

32.58

4.3

32.51

4.3

9

4.4

6.35

4.4

6.46

4.4

10

4.5

20.46

4.5

20.35

4.5

11

4.6

3.37

4.6

3.20

4.6

12

5.5

4.06

5.5

3.78

5.5

13

5.7

2.36

5.7

2.29

5.7

14

6.2

8.19

6.2

9.24

6.2

15

6.3

1.55

6.3

1.91

6.3

16

6.4

5.80

6.4

5.73

6.4

17

6.6

3.27

6.6

2.71

6.6

The capacity factor was calculated from the retention time of each peak of test item. The partition coefficient of each peak of test item was then calculated using the regression equation of regression line and was given as the average value of two measured values. Because the retention time of peak 15,peak 16 and peak 17 was longer than that of DDT (log Pow = 6.5), whose retention time was the maximum, the log Pow were recalculated by extrapolation.

It is judged that all of the test results are valid because the difference between two measured values of each peak was within ±0.1. The log Pow of the test item ranged from 1.9-6.6. The majority of the test item had a log Pow equal to or below 4.5.

The partition coefficient (log Pow) of peaks 1 to 10 (total peak area 71%) is≤4.5

The partition coefficient (log Pow) of peaks 11 to 17 (total peak area 29%) is >4.5

Applicant's summary and conclusion