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EC number: 947-862-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 - 21 Dec 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- 2011
- GLP compliance:
- yes
Test material
- Reference substance name:
- Fatty acids C8-18 (even numbered), mono- and diesters with sucrose
- EC Number:
- 947-862-2
- Molecular formula:
- not applicable, substance is UVCB
- IUPAC Name:
- Fatty acids C8-18 (even numbered), mono- and diesters with sucrose
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: All test concentrations incl. control
- Sampling method: Samples were taken at the beginning and end of exposure period.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test sample (0.0800 g) was dissolved in medium (800 mL) to prepare the stock solution of 100 mg/L. Required volumes of the stock solution and medium were mixed and stirred in a preparation container to prepare the test solution which was divided into each test vessel. The 100 mg/L test solution was prepared by direct addition.
- Eluate: no
- Differential loading: no (except for 100 mg/L)
- Controls: yes, test medium control
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: ATCC 22662
- Source (laboratory, culture collection): supplied by American Type Culture Collection, USA
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22.8 - 23.0 °C
- pH:
- 7.7 - 8.0
- Nominal and measured concentrations:
- nominal: control, 1.00, 3.16, 10.0, 31.6, 100 mg/L
measured: < LOQ, 0.98, 2.69, 8.47, 27.9, 93.6 mg/L (geometric mean) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Sterilized 300 mL Erlenmeyer flask (with gas-permeable Silicosen ®)
- Material, size, headspace, fill volume: glass, 300 mL, headspace: 200 mL, fill volume: 100 mL
- Aeration: constantly shaken on a rotary shakter (100 rpm)
- Initial cells density: 0.75E+04 cells/mL (equivalent to 56 Chlorophyll fluorescence units)
- Control end cells density: No cell density given (3900 Chlorophyll fluorescence units (mean))
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes (prepared in purified water)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: purified tap water
- Culture medium different from test medium: no
- Intervals of water quality measurement: Water quality parameters were measured regularly (temperature, pH.
OTHER TEST CONDITIONS
- Sterile conditions: yes
- Adjustment of pH: no
- Photoperiod: constant illumination
- Light intensity and quality: 90 µmol*m-2*s-1 (wavelength: 400 - 700 nm)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Chlorophyll measurement: Every 24 h the Chlorophyll content was measured using a spectrofluorometer to determine the Chlorophyll fluorescence value.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.16
- Range finding study
- Test concentrations: 1.00, 3.20, 10.0, 32.0, 100 mg/L
- Results used to determine the conditions for the definitive study: -2.1% inhibition at 1 mg/L, 5% inhibition at 3.20 mg/L, 13% inhibition at 10.0 mg/L, 17% inhibtion at 32 mg/L, 28% inhibition at 100 mg/L (inhibition values after 72 h). - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate was tested regularly (last test: 25 - 28 Dec 2017)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: All exposure levels were clear and colorless. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50 (72 h): 1.3 mg/L (95% CI: 0.61 - 1.4 mg/L) - Reported statistics and error estimates:
- Bartlett's test, One-way ANOVA, Dunnett's multiple comparison test
Any other information on results incl. tables
The measured test concentrations were in the range of 80 - 120% of nominal (see Table 2). Thus, it is justified that the effect concentrations were based on the nominal concentrations.
Table 1: Growth rate and growth inhibition rate
Nominal concentration [mg/L] |
Vessel No. |
Growth rate (0-3 d) |
Growth inhibition rate [%] |
Control |
A |
1.41 |
- |
B |
1.40 |
- |
|
C |
1.38 |
- |
|
D |
1.43 |
- |
|
E |
1.42 |
- |
|
F |
1.47 |
- |
|
Mean |
1.42 |
- |
|
S.D. |
0.0302 |
- |
|
1.00 |
A |
1.47 |
-4.0 |
B |
1.45 |
-2.0 |
|
C |
1.54 |
-8.6 |
|
Mean |
1.49 |
-4.9 |
|
S.D. |
0.0486 |
3.4 |
|
3.16 |
A |
1.41 |
0.61 |
B |
1.40 |
0.97 |
|
C |
1.48 |
-4.1 |
|
Mean |
1.43 |
-0.85 |
|
S.D. |
0.0405 |
2.9 |
|
10.0 |
A |
1.29 |
9.2 |
B |
1.23 |
13 |
|
C |
1.32 |
7.2 |
|
Mean |
1.28 |
9.9 |
|
S.D. |
0.0433 |
3.1 |
|
31.6 |
A |
1.15 |
19 |
B |
1.13 |
21 |
|
C |
1.24 |
13 |
|
Mean |
1.17 |
17 |
|
S.D. |
0.0582 |
4.1 |
|
100 |
A |
1.04 |
26 |
B |
1.00 |
29 |
|
C |
1.15 |
19 |
|
Mean |
1.07 |
25 |
|
S.D. |
0.0775 |
5.5 |
n.d.: < 0.541 mg/L
Table 2: Analytical results
Nominal concentration [mg/] |
Measured concentration [mg/L] (%) |
||
0 h |
72 h |
Geometric mean |
|
Control |
Not detected |
Not detected |
- |
1.00 |
0.980 (98.0) |
0.980 (98.0) |
0.980 (98.0) |
3.16 |
2.85 (90.2) |
2.53 (80.2) |
2.69 (85.1) |
10.0 |
8.95 (89.5) |
8.01 (80.1) |
8.47 (84.7) |
31.6 |
29.6 (93.7) |
26.2 (83.0) |
27.9 (88.2) |
100 |
101 (101) |
87.2 (87.2) |
93.6 (93.6) |
Table 3: Validity criteria for OECD 201.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The biomass in the control cultures should have increased exponentially by a factor of at least 16 within the 72-hour test period. |
Factor of > 64 |
yes |
The mean coefficient of variation for section-by-section specific growth rates (days 0-1, 1-2 and 2-3, for 72-hour tests) in the control cultures must not exceed 35% |
11% |
yes |
The coefficient of variation of average specific growth rates during the whole test period in replicate control cultures must not exceed 7% in tests with Pseudokirchneriella subcapitata and Desmodesmus subspicatus. For other less frequently tested species, the value should not exceed 10%. |
2.1% |
yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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