Registration Dossier
Registration Dossier
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Reaction mass of 3,3'-(1E,1'E)-(4,4'-(6-(bis(2-hydroxyethyl)amino)-1,3,5-triazine-2,4-diyl)bis(azanediyl)bis(3-methoxy-4,1-phenylene))bis(diazene-2,1-diyl)dibenzenesulfonic acid and disodium 3,3'-[[6-[bis(2-hydroxyethyl)amino]-1,3,5-triazine-2,4-diyl]bis[imino(3-methoxy-4,1-phenylene)azo]]bis[benzenesulphonate]
EC number: 943-704-1 | CAS number: -
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
Not harmful/toxic to aquatic invertebrates up to the limit of solubility in the test medium, i.e. EC50 (48h) > 78 mg/l (meas. geom. mean).
Key value for chemical safety assessment
Additional information
No data on target substance was available, thus a read across approach was followed. Available data on Similar Substance 01 was used. Details on the read across are attached in section 13.
Similar Substance 01 was tested for acute toxicity to Daphnia magna in a 48-h static test, according to OECD guideline 202 (2004). The only nominal concentration tested was 100 mg/l and a control. In pre-experiments, partial precipitation of test item was noted during the test period, presumably due to formation of complexes with ions of test water. Therefore, the hardness of test water for the main study was lowered to the minimum hardness of 140 mg/l (as CaCO3) recommended by the OECD test guideline to minimise precipitation.
At start of the test, the measured concentration of test item in test medium was 82 % of the nominal concentration. At the end of the test, 74 % of nominal concentration was found in test medium. The slight decrease of test item concentration in test medium during test period was caused by precipitation and sedimentation of part of test item. At the end of the test, part of test item was on the bottom of test vessels. Thus, the maximum solubility of test item in test medium under test conditions was obviously exceeded.
In the control and at nominal concentration of 100 mg/l (mean measured concentration of 78 mg/l), no immobilised test organisms were observed during the test period of 48 hours. Therefore, the 48-h NOEC was determined to be at least 100 mg/l (mean measured 78 mg/l). The 48-hour NOEC and the 48-h might even be higher, but higher concentrations of test item could not be tested due to the limited solubility of test item in test water.
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