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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2006
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The concentration of the test substance was not stable during the test. The test item likely absorbed to the glass wall or the test organisms.
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
The test concentration was measured at 0, 24 and 48 hours.
The daphnia were checked for immobilization after 24 and 48 hours.
Vehicle:
yes
Details on test solutions:
0, 1.0, 1.8, 3.2, 5.6 and 10 µg/L, applied dissolved in acetone.
Test organisms (species):
Daphnia magna
Details on test organisms:
6 to 24 hours old daphnia have been used. The strain was daphnia magna Straus.
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
no
Hardness:
Reconstituted test water: analytical grade salts were dissolved to obtain water hardness of 2.5 mmol l-1.
Test temperature:
21.8 - 22.1 °C
pH:
The pH ranged from 7.05 - 7.81.
Dissolved oxygen:
The dissolved oxygen was between 5.8 and 6.5 mg/L.
Salinity:
not applicable
Nominal and measured concentrations:
Nominal: 0, 1.0, 1.8, 3.2, 5.6 and 10 µg/L.
Measured: Levels could not be detected in any of the samples, presumably due to sorption of the test substance to the glass walls or to the test organisms.
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
ca. 3.2 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC10
Effect conc.:
ca. 5.6 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
IC50
Effect conc.:
ca. 4.6 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 1.1 - 5.6
Results with reference substance (positive control):
Not appplicable.
Reported statistics and error estimates:
Reference is made to Appendix B of the study report.

Nominal test concentration

No. of daphnia tested

No. of immobilized daphnia

% of of immobilized daphnia

[ug l-1]

 

24h

48h

24h

48h

0 (control)

20

0

0

0

0

0 (solvent control)

20

0

0

0

0

1.0

20

0

0

0

0

1.8

20

0

4

0

20

3.2

20

0

4

0

20

5.6

20

5

14

25

70

10

20

8

20

40

100

Validity criteria fulfilled:
yes
Conclusions:
Despite some deficiencies, the results are in line with other available data on d-Phenothrin.
EC0 = 3.2 µg/L
EC10 = 5.6 µg/L
IC50 = 4.6 µg/L
Executive summary:

EC0 = 3.2 µg/L

EC10 = 5.6 µg/L

IC50 = 4.6 µg/L

Description of key information

The acute toxicity to daphnia magna upon exposure during 48 hours was investigated ina GLP compliant OECD 202 study. The immobilisation IC50 value was found being 4.6 µg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
4.6 µg/L

Additional information