Registration Dossier

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2006
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The concentration of the test substance was not stable during the test. The test item likely absorbed to the glass wall or the test organisms.
Reason / purpose for cross-reference:
reference to other study
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
The test concentration was measured at 0, 24, 48 an 96 hours.
The fish were checked for mortality after 2, 24, 48, 72 and 96 hours.
Vehicle:
yes
Details on test solutions:
0, 10, 17.8, 31.6, 56.2 and 100 µg/L, applied dissolved in ethanol and water.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Juvenile fish of the same age and size have been used. They had been kept for 14 days under stable conditions.During the holding period, they were fed up to 48 hours before the test.
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
No
Hardness:
Reconstituted test water: analytical grade salts were dissolved to obtain water hardness of 2.5 mmol l-1.
Test temperature:
21.7 - 23.2 °C
pH:
The pH ranged from 6-9 and varied not more than 0.4 units.
Dissolved oxygen:
The dissolved oxygen was ≥6.6 mg/L.
Salinity:
not applicable
Nominal and measured concentrations:
Nominal: 0, 10, 17.8, 31.6, 56.2 and 100 µg/L.
Measured: Levels could be detected only in 3 samples, presumably due to sorption of the test substance to the glass walls or to the test organisms.
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel:
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 hours
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
ca. 10 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
EC10
Effect conc.:
ca. 31.6 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
ca. 55.9 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: not available
Details on results:
While lowest mortality was observed at the lowest dose, and highest mortality at the highest dose, there was no consistent dose-response relationship. The intermediate doses showed lower mortality than the low dose, as is reported in the table below under "Any other information on results incl. tables".
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
Reference is made to Appendix C of the study report.
Sublethal observations / clinical signs:

 

Nominal test concentration [ug l-1]

No. of exposed fish

2h

24h

48h

72h

96h

0 (control)

7

0

0

0

0

0

0 (solvent control)

7

0

0

0

0

0

10

7

0

0

0

0

0

17.8

7

0

4

4

5

5

31.6

7

0

0

0

1*

2*

56.2

7

0

2

2

2*

3*

100

7

0

4

7

7

7

* All of the survivors showed signs of distress such as apathy and lateral swim and some were lying on one side

Validity criteria fulfilled:
yes
Conclusions:
Despite some deficiencies, the results are in line with other available data on d-Phenothrin.
EC0 = 10 µg/L
EC10 = 31.6 µg/L
LC50 = 55.9 µg/L
Executive summary:

EC0 = 10 µg/L

EC10 = 31.6 µg/L

LC50 = 55.9 µg/L

Description of key information

The acute aquatic toxicity to fish was found being 55.9 µg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
55.9 µg/L

Additional information