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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Justification for type of information:
data is from NTRL reports

Data source

Reference
Reference Type:
secondary source
Title:
Initial submission: The acute of toxicity certain compounds of molybdenum report to the International Molybdenum Association with attachments and cover letter dated 062891
Author:
International Molybdenum association
Year:
1991
Bibliographic source:
NTRL submission report -OTS0000811, last updated 1991

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
Principles of method if other than guideline:
The dermal irritation potential of Ammonium molybdate was assessed in rabbits according to OECD 404 Guidelines
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid
Details on test material:
- Name of test material (as cited in study report): ammonium molybdate(VI)
- Molecular formula: MoO4.2H4N
- Molecular weight: 196.0132 g/mol
- Smiles notation: [Mo](=O)(=O)([O-])[O-].[NH4+].[NH4+]
- InChl: 1S/Mo.2H3N.4O/h;2*1H3;;;;/q;;;;;2*-1/p+2
- Substance type: Inorganic
- Physical state: Solid
Specific details on test material used for the study:
- Name of test material (as cited in study report): ammonium molybdate(VI)
- Molecular formula: MoO4.2H4N
- Molecular weight: 196.0132 g/mol
- Smiles notation: [Mo](=O)(=O)([O-])[O-].[NH4+].[NH4+]
- InChl: 1S/Mo.2H3N.4O/h;2*1H3;;;;/q;;;;;2*-1/p+2
- Substance type: Inorganic
- Physical state: Solid

Test animals

Species:
rabbit
Strain:
New Zealand White
Details on test animals and environmental conditions:
TEST ANIMALS
-Sex: Female
- Source: A. Smith, Warlingham. Surrey
- Age at study initiation: 9 to 12 weeks of age
- Weight at study initiation: 2.2 to 2.7 kg
- Fasting period before study:
- Housing: The rabbits were individually housed in metal cages with perforated floors in Building
- Diet (e.g. ad libitum): SDS Standard Rabbit Diet
- Water (e.g. ad libitum):Tap water
- Acclimation period: The rabbits selected for the study were all acclimated to the laboratory environment


ENVIRONMENTAL CONDITIONS
- Temperature (°C):19 deg C
- Humidity (%):Relative humidity:30-70%
- Air changes (per hr): 19 air changes per hour
- Photoperiod (hrs dark / hrs light): 12 hours of artificial light in each 24 hour period.

Test system

Type of coverage:
semiocclusive
Preparation of test site:
clipped
Vehicle:
water
Controls:
not specified
Amount / concentration applied:
0.5 gm
Duration of treatment / exposure:
4 hours
Observation period:
Day 1(approximately 30 minutes after removal of the patches) and on Days 2, 3 and 4.
Number of animals:
6 female rabbits
Details on study design:
TEST SITE
- Area of exposure: dorso-lumbar region
- % coverage: 10 cm square patch
- Type of wrap if used: Elastoplast

REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes
- Time after start of exposure: At the end of the exposure period, the semi-occlusive dressing and gauze pad were removed and the treatment site was washed using water to remove any residual test substance.
SCORING SYSTEM:
Grading and scoring of the dermal reactions were performed using the prescribed numerical scoring system.

Results and discussion

In vitro

Other effects / acceptance of results:
no data available

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
mean
Time point:
72 h
Score:
0
Max. score:
4
Reversibility:
not specified
Remarks on result:
no indication of irritation
Irritation parameter:
edema score
Basis:
mean
Time point:
72 h
Score:
0
Max. score:
4
Reversibility:
not specified
Remarks on result:
no indication of irritation
Irritant / corrosive response data:
No dermal irritation observed

Any other information on results incl. tables

Table: Dermal reactions elicited by Ammonium Molybdate

Rabbit

number

Sex

 

E =Erythema

o =Oedema

Day

1*

2

3

4

1

female

E

0

0

0

0

O

0

0

0

0

2

Female

E

0

0

0

0

O

0

0

0

0

3

Female

E

0

0

0

0

O

0

0

0

0

4

Female

E

0

0

0

0

O

0

0

0

0

5

Female

E

0

0

0

0

O

0

0

0

0

6

Female

E

0

0

0

0

O

0

0

0

0

*-Approximately 30 minutes after removal of dressing

Applicant's summary and conclusion

Interpretation of results:
other: not irritating
Conclusions:
A single semi-occlusive application of Ammonium Molybdate to intact rabbit skin for four hours elicited no dermal irritation.
The overall irritation index after 4 days of observation was 0.0.
Hence, Ammonium molybdate can be considered not irritating to skin.
Executive summary:

A study was conducted to assess the irritation potential of ammonium molybdate. The study was conducted as per OECD 404 Guidelines.

0.5 gm of test chemical was applied to the dorso-lumbar region exposing an area of skin approximately 10 cm square under 2.5 cm square gauze pad moistened with 0.5 ml distilled water to one intact skin site of 6 female New Zealand White rabbits.Each treatment site was occluded with Elastoplast elastic adhesive dressing for a four hour period.At the end of the exposure period, the semi-occlusive dressing and gauze pad were removed and washed and animal were scored in accordance with edema erythema formation.Examination of the treated skin was made on Day 1 (i.e. approximately 30 minutes after removal of the patches) andon Days 2, 3 and 4.There was no response to treatment in any animal throughout the observation period.A single semi-occlusive application of Ammonium Molybdate to intact

rabbit skin for four hours elicited no dermal irritation.

The overall irritation index after 4 days of observation was 0.0.Hence, Ammonium molybdate can be considered not irritating to skin.