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EC number: 946-103-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to terrestrial plants
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to terrestrial plants: short-term (with study design considered suitable for long-term assessment)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014-04-08 to 2014-04-29
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on sampling:
- No analytical monitoring
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): The respective test item amount was weighed out for each test item concentration and mixed thoroughly with
quartz sand in a glass vessel (1 % of the entire soil amount). Afterwards the treated quartz sand was mixed carefully with the soil using a mixer to
ensure a homogeneous distribution of the test item. The treated soil was transferred to the test container and the seeds were sown.
- Control: Untreated soil with 1 % quartz sand - Species:
- Brassica napus
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Rape
- Plant family: Brassicaceae
- Variety: Kadore
- Source of seed: KWS Mais GmbH, D-37555 Einbeck, Germany
- Prior seed treatment/sterilization: No
- Seed storage: The seeds were stored dark at the test facility at room temperature (22 ± 10 °C) and protected from moisture until use. - Species:
- Avena sativa
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: Oats
- Plant family: Gramineae
- Variety: Ivory
- Source of seed: NORDSAAT SAATZUCHT GMBH, 38895 Langenstein, Germany
- Prior seed treatment/sterilization: No
- Seed storage: The seeds were stored dark at the test facility at room temperature (22 ± 10 °C) and protected from moisture until use. - Species:
- Glycine max (G. soja)
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Soybean
- Plant family: Leguminosae
- Variety: Merlin
- Source of seed: Saatbau Linz, A-4021 Linz, Austria
- Prior seed treatment/sterilization: No
- Seed storage: The seeds were stored dark at the test facility at room temperature (22 ± 10 °C) and protected from moisture until use. - Test type:
- seedling emergence toxicity test
- Study type:
- laboratory study
- Substrate type:
- natural soil
- Limit test:
- no
- Total exposure duration:
- 21 d
- Test temperature:
- nominal: 22 +/- 10 °C
actual: 13 - 21 °C - pH:
- 5.31 +/- 0.01 (natural soil)
- Moisture:
- - relative humidity: nominal: 70 +/- 25 %, actual: 58 - 100 %
- Details on test conditions:
- TEST SYSTEM
- Testing facility: Dr.U.Noack-Laboratorien
- Test container (type, material, size): Plastic container (standard flower pots) with a diameter of ca. 12 cm
- Amount of soil:
Control and 1000 mg/kg DW: 16.94 kg, divided into two parts, each 8.47 kg
400 - 160 - 64 - 25.6 mg/kg DW: 12.72 kg, divided into two parts, each 6.36 kg
- Method of seeding: Test containers were filled each with the same amount of treated soil (see application technique) and seeding holes were made
with a seedling pistil. One seed was given in each hole and covered with soil. During cultivation the test containers were bottom watered and
fertilized as necessary.
- No. of seeds per container: 5
- No. of replicates per treatment group: 8
- No. of replicates per control: 8
SOURCE AND PROPERTIES OF SUBSTRATE (if soil): A 2:1 mixture of natural soil LUFA 2.2 (batch number: Sp2.20214) and quartz sand (12a) was used.
- Geographic location: Soil collected by LUFA Speyer (67346 Speyer, Germany), quartz sand collected by Dörentrup Quarz GmbH Co. KG (31089
Duingen, Germany)
- Pesticide use history at the collection site: No pesticide use for the last five years.
- Soil texture (if natural soil)
- grain size: = 2 mm
- Soil taxonomic classification: loamy sand (IS)
- Soil classification system: German DIN
- Organic carbon (%): 0.73
- CEC: 9.9 +/- 0.7 mval/100 g
- Storage (condition, duration): The soil has been stored at the test facility at green house conditions until use.
NUTRIENT MEDIUM (if used)
- Description:
Fertilizer: HAKAPHOS® SOFT SPEZIAL 16+8+22(+3) (0.5 ‰)
Origin: GRÜNES LANDHAUS, St. Godehard-Str. 23, 31139 Hildesheim, Germany
Nutrient Concentration [%]
Ammonium nitrogen 9.7
Nitrate nitrogen 6.3
P2O5 8
Potassium oxide 22
Magnesium oxide 3
Boron 0.03
Iron (Chelate) 0.15
Copper 0.02
Manganese 0.05
Molybdenum 0.005
Zinc 0.015
GROWTH CONDITIONS
- Photoperiod: 16 h
- Light source: Special-fluorescent tube Fluora and Daylight (OSRAM)
- Light intensity and quality: nominal: > 3500 lx, actual: 4954 ± 525
- Day/night temperatures: 22 +/- 10 °C, actual: 13 - 21 °C
- Relative humidity (%): 70 +/- 25, actual: 58 - 100
- Watering regime and schedules: Bottom watering of the test containers with nutrient solution as necessary.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
During the 21 day observation period the plants were observed every 7 days for number of emerged seedlings, number of dead plants and visual
phytotoxic effects. The rating of the treated plants was done in relation to the control plants. Observations included all variations, either inhibitory
or stimulatory, between the treated test replicates and the untreated control replicates. Such variations were phytotoxic symptoms (chlorosis,
necrosis, wilting), formative effects and growth and development rates. At the end of the study shoot height and shoot fresh weight were measured.
Day -1: Determination of the WHC and the dry weight of the soil
Day 0 (Experimental starting): Application and sowing as specified above.
Every working day: The test containers were bottom watered and fertilised as needed.
Day 7, 14, 21: The replicates were observed for visual phytotoxic effects number of emerged seedlings and number of dead plants.
Test end (day 21): Final shoot heights and shoot fresh weight measurements were done.
- Phytotoxicity rating system (if used):
(1) Alteration of form and appearance (deformation)
(1.1) Curling / twisting / sticking or other deformation of the leafs
(1.2) Change of appearance
(1.3) Shoot deformations
(1.4) Wilting
(1.5) Leaf shedding
(1.6) Shedding of the leaf apex
(1.7) Stunted growth
(1.8) Lacking of cotyledons
(2) Discolouration of the leafs
(2.1) Lighter green (2.2) Darker green
(2.3) Chlorosis (2.3.1) Spots
(2.3.2) Whole leaf
(2.3.3) Veins
(2.3.4) Between veins
(2.3.5) Marginal
(2.4) Loss of colour
(2.5) Discolouration
(3) Alteration of the leaf cells
(3.1) Necrosis (3.1.1) Spots
(3.1.2) Marginal
(3.1.3) Apical
(3.1.4) Between veins
(3.1.5) Whole leaf
(E) Dead plant (Number of dead plants)
Assessment of the Effects
- = no effects observed / normal growth
+/- = slight effects
+ = medium effects
++ = strong effects
VEHICLE CONTROL PERFORMED: no
TEST CONCENTRATIONS
- Range finding study
Results of the Preliminary Test: Inhibition of Shoot Fresh Weight and Emergence on day 21
Test
concentration Inhibition of shoot fresh weight [%] Inhibition of emergence
[%]
[mg/kg DW] Oats Rape Soybean Oats Rape Soybean
10 4 4 -2 5 -6 -7
100 5 18 -2 0 0 -7
1000 8 16 8 5 -11 -7
- Nominal and measured concentrations:
- nominal:
Oats: 1000 mg/kg DW (dry weight)
Rape, soybean: 1000 - 400 - 160 - 64 - 25.6 mg/kg DW (dry weight) - Reference substance (positive control):
- no
- Species:
- Avena sativa
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot fresh weight, shoot height)
- Species:
- Brassica napus
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot fresh weight, shoot height)
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot fresh weight, shoot height)
- Details on results:
-
SEEDLING EMERGENCE
The percent inhibition of shoot height, shoot fresh weight and rate of emergence is given in the tables below. The tabulated results represent
rounded values calculated on the exact raw data.
Oats: Inhibition [%] of Shoot Height, Shoot Fresh Weight and Rate of Emergence
Test concentration [mg/kg DW] Shoot height [cm] Inhibition [%] Shoot fresh weight [mg] Inhibition [%] Rate of emergence [%] Inhibition [%]
Control 40.5 - 1041 - 98 -
1000 41.8 -3 1029 1 98 0
Rape: Inhibition [%] of Shoot Height, Shoot Fresh Weight and Rate of Emergence
Test concentration [mg/kg DW] Shoot height [cm] Inhibition [%] Shoot fresh weight [mg] Inhibition [%] Rate of emergence [%] Inhibition [%]
Control 15.3 - 1628 - 95 -
25.6 14.8 3 1492 8 93 2
64 14.7 4 1471 10 95 0
160 14.7 4 1487 9 93 2
400 14.5 5 1459 10 95 0
1000 14.5 5 1380 15 95 0
Soybean: Inhibition [%] of Shoot Height, Shoot Fresh Weight and Rate of Emergence
Test concentration [mg/kg DW] Shoot height [cm] Inhibition [%] Shoot fresh weight [mg] Inhibition [%] Rate of emergence [%] Inhibition [%]
Control 28.9 - 2199 - 100 -
25.6 27.5 5 2174 1 98 2
64 26.9 7 2141 3 98 2
160 27.0 7 2028 8 100 0
400 27.5 5 2057 6 100 0
1000 28.6 1 2225 -1 100 0
Negative values = promoted growth - Results with reference substance (positive control):
- No reference substance tested
- Reported statistics and error estimates:
- The percent inhibition of biomass growth (shoot height, shoot fresh weight) and rate of emergence were calculated in relation to the control.
One Way Analysis of Variance (ANOVA) was carried out for the determination of statistically significant differences compared to control replicates. When
running a One Way Analysis of Variance a Normality test and an Equal Variance test were done first. P-values for both Normality and Equal Variance test are
0.05. The -value for ANOVA (acceptable probability of incorrectly concluding that there is a difference) is =0.05. - Validity criteria fulfilled:
- yes
- Conclusions:
- After 21 days no test item related phytotoxic effects were noted for all tested plant species at test concentrations up to 1000 mg/kg DW.
No test item related effects > 15 % were observed on the shoot height, the shoot fresh weight and the rate of emergence of all tested plant species.
Therefore the EC50 -Value is > 1000 mg/kg soil DW for shoot height, shoot fresh weight and rate of emergence for all tested plant species.
The NOEC was determined to be 1000 mg/kg DW for shoot height, shoot fresh weight and rate of emergence for all tested plant species. (see table below).
NOEC Values of Shoot Height, Shoot Fresh Weight and Rate of Emergence
NOEC [mg/kg DW]
Species Shoot height Shoot fresh weight Rate of emergence
Oats 1000 1000 1000
Rape 1000 1000 1000
Soybean 1000 1000 1000 - Executive summary:
The phytotoxicity of the test item Celitement (batch number: Not specified) to three terrestrial plant species was determined in a seedling emergence test over a period of 21 days, from 2014 -04 -08 to 2014 -04 -29 according to OECD 208 (July 2006) at Dr.U.Noack-Laboratorien,
31157 Sarstedt, Germany.
Test systems were one monocotyledon, oats (Gramineae), and two dicotyledons, rape (Brassicaceae) and soybean (Leguminosae).
The test was conducted for the plant species oats with the limit concentration1000 mg/kg soil dry weight (DW)and for the plant species rape and soybean with the test concentrations of 1000 - 400 - 160 - 64 - 25.6 mg/kg soil dry weight (DW).
The test item was incorporated into the soil in which the seeds were sown afterwards. The test containers were bottom watered with nutrient solution as needed. The toxic effects of the test item were determined on day 7, 14 and 21 by visual observations (number of emerged seedlings and phytotoxic effects) and on day 21 by shoot height and shoot fresh weight determination.
After 21 days no test item related phytotoxic effects were determined for all tested plant species up to the test concentration 1000 mg/kg DW.
No inhibitions > 15 % were determined for shoot height, shoot fresh weight and rate of emergence for all tested plant species (see tables below). Therefore the EC50-Value is > 1000 mg/kg soil DW for shoot height, shoot fresh weight and rate of emergence for all tested plant species.
The NOEC was determined to be 1000 mg/kg DW for shoot height, shoot fresh weight and rate of emergence for all tested plant species (see table below).
Oats: Inhibition [%] of Shoot Height, Shoot Fresh Weight and Rate of Emergence
Test concentration
[mg/kg DW]Inhibition [%]
Shoot
heightShoot fresh weight
Rate of emergence
1000
-3
1
0
Negative values = promoted growth
Rape: Inhibition [%] of Shoot Height, Shoot Fresh Weight and Rate of Emergence
Test concentration
[mg/kg DW]Inhibition [%]
Shoot
heightShoot fresh weight
Rate of emergence
25.6
3
8
2
64
4
10
0
160
4
9
2
400
5
10
0
1000
5
15
0
Soybean: Inhibition [%] of Shoot Height, Shoot Fresh Weight and Rate of Emergence
Test concentration
[mg/kg DW]Inhibition [%]
Shoot
heightShoot fresh weight
Rate of emergence
25.6
5
1
2
64
7
3
2
160
7
8
0
400
5
6
0
1000
1
-1
0
NOEC Values of Shoot Height, Shoot Fresh Weight and Rate of Emergence
NOEC [mg/kg DW]
Species
Shoot height
Shoot fresh weight
Rate of emergence
Oats
1000
1000
1000
Rape
1000
1000
1000
Soybean
1000
1000
1000
Reference
Visual Phytotoxicity on Day 21
Oats: Visual Observations and Number of Dead Plants on Day 21
Test |
Phyto- |
Phytotoxic effects |
|||||||
Replicate |
|||||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
||
control |
– |
- |
- |
- |
- |
- |
- |
- |
- |
1000 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
Rape: Visual Observations and Number of Dead Plants on Day 21
Test |
Phyto- |
Phytotoxic effects |
|||||||||||||||||
Replicate |
|||||||||||||||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
||||||||||||
control |
– |
- |
- |
- |
- |
- |
- |
- |
- |
||||||||||
25.6 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
||||||||||
64 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
||||||||||
160 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
||||||||||
400 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
||||||||||
1000 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
Soybean: Visual Observations and Number of Dead Plants on Day 21
Test |
Phyto- |
Phytotoxic effects |
|||||||
Replicate |
|||||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
||
control |
– |
- |
- |
- |
- |
- |
- |
- |
- |
25.6 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
64 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
160 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
400 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
1000 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
- = no effects observed / normal growth
Description of key information
The NOEC was determined to be 1000 mg/kg DW for shoot height, shoot fresh weight and rate of emergence for all tested plant species.
Key value for chemical safety assessment
- Short-term EC50 or LC50 for terrestrial plants:
- 1 000 mg/kg soil dw
- Long-term EC10, LC10 or NOEC for terrestrial plants:
- 1 000 mg/kg soil dw
Additional information
After 21 days no test item related phytotoxic effects were noted for all tested plant species at test concentrations up to 1000 mg/kg DW. No test item related effects > 15 % were observed on the shoot height, the shoot fresh weight and the rate of emergence of all tested plant species. Therefore the EC50 -Value is > 1000 mg/kg soil DW for shoot height, shoot fresh weight and rate of emergence for all tested plant species. The NOEC was determined to be 1000 mg/kg DW for shoot height, shoot fresh weight and rate of emergence for all tested plant species. (see table below). NOEC Values of Shoot Height, Shoot Fresh Weight and Rate of Emergence NOEC [mg/kg DW] were 1000 for all tested plant species.
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