Sodium 4-chloro-3-nitrobenzenesulphonate

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

data is from experimental reports

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Principles of method if other than guideline:

The objective of the study was to determine the cutaneous toxicity of Sodium 4-chloro-3-nitrobenzenesulphonate

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Sodium 4-chloro-3-nitrobenzenesulphonate

EC Number:

241-680-0

EC Name:

Sodium 4-chloro-3-nitrobenzenesulphonate

Cas Number:

17691-19-9

Molecular formula:

C6H3ClNNaO5S

IUPAC Name:

sodium 4-chloro-3-nitrobenzene-1-sulfonate

Test material form:

solid: particulate/powder

Details on test material:

- Name of the test material: Sodium 4-chloro-3-nitrobenzenesulphonate
- IUPAC Name : sodium 4-chloro-3-nitrobenzene-1-sulfonate
- Molecular formula: C6H4ClNO5SNa
- Molecular weight: 259.601 g/mol
- Smiles: S(=O)(=O)([O-])c1cc([N+]([O-])=O)c(Cl)cc1.[Na+]
- InChI: 1S/C6H4ClNO5S.Na/c7-5-2-1-4(14(11,12)13)3-6(5)8(9)10;/h1-3H,(H,11,12,13);/q;+1/p-1
- Substance type: Organic
- Physical state: Solid powder (Off white to pale yellow)

Specific details on test material used for the study:

- Name of the test material: Sodium 4-chloro-3-nitrobenzenesulphonate
- IUPAC Name : sodium 4-chloro-3-nitrobenzene-1-sulfonate
- Molecular formula: C6H4ClNO5SNa
- Molecular weight: 259.601 g/mol
- Smiles: S(=O)(=O)([O-])c1cc([N+]([O-])=O)c(Cl)cc1.[Na+]
- InChI: 1S/C6H4ClNO5S.Na/c7-5-2-1-4(14(11,12)13)3-6(5)8(9)10;/h1-3H,(H,11,12,13);/q;+1/p-1
- Substance type: Organic
- Physical state: Solid powder (Off white to pale yellow)

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Sex: Male and female
- Source: Hacking and Churchill Limited, Huntingdon, England
- Age at study initiation: no data available
- Weight at study initiation:
- Housing:individually in stainless steel cages
- Diet (e.g. ad libitum): Ssniff K Pallets, ad libitum (Ssniff Experimental animal diet GmbH, 4770 Soest)
- Water (e.g. ad libitum): Drinking water ad libitum from plastic bottles with steel nipples
- Acclimation period: no data available

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 deg C
- Humidity (%): 60%
- Air changes (per hr): no data available
- Photoperiod (hrs dark / hrs light):12-hour light-dark rhythm

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

water

Controls:

not specified

Amount / concentration applied:

500 mg of the substance were dissolved with 0.1 ml of double-distilled water

Duration of treatment / exposure:

4 hours

Observation period:

(1h), 24, 48, 72 hours and, if necessary, 7 days after treatment for erythema on the cutaneous

Number of animals:

3 (1 male and 2 female)

Details on study design:

TEST SITE
- Area of exposure: dorsal and lateral parts of the trunk
- % coverage: 2.5 × 2.5 cm gauze swab Transfilmine. Combined Fixiarl
- Type of wrap if used: Transfilmine. Combined Fixiarl

REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes
- Time after start of exposure: After the end of the exposure time, the bare skin areas were rinsed with water and soap and dried.

OBSERVATION TIME POINTS
(indicate if minutes, hours or days) : The observation period lasted 72 hours or 7 days;
Each animal was observed at 1h, 24, 48, 72 hours and, if necessary, 7 days after treatment for erythema and edema

SCORING SYSTEM:
- Method of calculation: Skin lesions were scored according to the following OECD Guidelines on testing materials where appropriate responses were recorded.

Results and discussion

In vitro

Other effects / acceptance of results:

no data available

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

72 h

Score:

0

Reversibility:

not specified

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

All rabbits in which the test substance was applied to the skin, negative effects were observed

Any other information on results incl. tables

Table: Acute Dermal Irritation in Rabbits

(According to OECD 404 Guidelines)

Animal number	Sex	Erythema					Edema					Skin fold thickness
		1h	24h	48h	72h	7 days	1h	24h	48h	72h	7 days
135	Male	0	0	0	0	-	0	0	0	0	-	Not measured
194	Female	0	0	0	0	-	0	0	0	0	-	Not measured
195	Female	0	0	0	0	-	0	0	0	0	-	Not measured
Mean		0	0	0	0	-	0	0	0	0	-

Applicant's summary and conclusion

Interpretation of results:

other: Not irritating

Conclusions:

Negative effects were observed in all the rabbits after 72 hours of observation. The mean irritation score was 0.0 after 72 hours. Hence, Sodium 4-chloro-3-nitrobenzenesulphonate can be considered to be not irritating to skin.

Executive summary:

The objective of the study was to determine the cutaneous toxicity of Sodium 4-chloro-3-nitrobenzenesulphonate. The study was performed as per OECD 404 Guidelines. 3(1 male and 2 female) New Zealand White rabbits were used. For the determination of the acute irritative effect, the dorsal and lateral parts of the trunk were shaved 16-24 hours prior to treatment with an electric cutting machine. The test substance was administered as an 83% aqueous solution. In addition, 500 mg of the substance were dissolved with 0.1 ml of double-distilled water. The aqueous solution was spread on a flax of about 6 cm 2 on the gastric dorsal skin, covered with a 2.5 × 2.5 cm gauze swab Transfilmine Combined Fixiarl for four hours.During the exposure time, the rabbits were housed individually in separate cages. After the end of the exposure time, the bare skin areas were rinsed with water and soap and dried. The observation period lasted 72 hours or 7 days. Each animal was observed at 1h, 24, 48, 72 hours and, if necessary, 7 days after treatment for erythema and edema. Skin lesions were scored according to the following OECD Guidelines on testing materials where appropriate responses were recorded.

Negative effects were observed in all the rabbits after 72 hours of observation. The mean irritation score was 0.0 after 72 hours. Hence,  Sodium 4-chloro-3-nitrobenzenesulphonate can be considered to be not irritating to skin.