Sodium 4-chloro-3-nitrobenzenesulphonate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

data is from experimental reports

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Acute Oral toxicity test was carried out to study the effects of Sodium 4-chloro-3-nitrobenzenesulphonate (17691-19-9) on rats.

GLP compliance:

not specified

Test type:

other: no data available

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Sodium 4-chloro-3-nitrobenzenesulphonate

EC Number:

241-680-0

EC Name:

Sodium 4-chloro-3-nitrobenzenesulphonate

Cas Number:

17691-19-9

Molecular formula:

C6H3ClNNaO5S

IUPAC Name:

sodium 4-chloro-3-nitrobenzene-1-sulfonate

Details on test material:

- Name of test material (IUPAC name): Sodium 4-chloro-3-nitrobenzenesulphonate
- Common name: 4-Chloro-3-nitrobenzenesulfonic acid, sodium salt
- Molecular formula: C6H3ClNNaO5S
- Molecular weight: 259.601 g/mol
- Smiles notation: S(=O)(=O)([O-])c1cc([N+]([O-])=O)c(Cl)cc1.[Na+]
- InChl: 1S/C6H4ClNO5S.Na/c7-5-2-1-4(14(11,12)13)3-6(5)8(9)10;/h1-3H,(H,11,12,13);/q;+1/p-1
- Substance type: Organic

Specific details on test material used for the study:

- Name of test material (as cited in study report):sodium 4–chloro–3–nitrobenzenesulphonate
- Molecular formula :C6H4NNaO5S
- Molecular weight :225.1556 g/mol
- Substance type:organic

Test animals

Species:

rat

Strain:

other: Wistar (SPF cpB)

Sex:

male/female

Details on test animals or test system and environmental conditions:

Details on test animal:
TEST ANIMALS:
- Source: winkelmann, Borchen
- Age at study initiation: 14 weeks old avg.
- Weight at study initiation: Initial weight of male animals was 161 g and the female rats had an average initial weight of 160 g.
- Fasting period before study: No data available
- Housing: The rats were kept conventionally in Makrolonkaefigen type III on dust-free wood granules in groups of 5 each animals.
- Diet (e.g. ad libitum): the feed : Altromin R 1324 was given ad libitum
- Water (e.g. ad libitum): water was provided ad libitum for approximately 16 hours and before until about 4 hours after application.
- Acclimation period: No data available

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 1.5 degrees Celsius
- Humidity (%):approx. 60 +/- 5%
- Air changes (per hr): No data available
- Photoperiod (hrs dark / hrs light): 2 hours of light / dark rhythm (artificial Lighting from 7 am to 7 pm CET)

IN-LIFE DATES: From: To: 25.8.1980 to 8.9.1980

Administration / exposure

Route of administration:

oral: unspecified

Vehicle:

water

Details on oral exposure:

Details on exposure:
VEHICLE
- Concentration in vehicle:5000mg/kg bw
- Amount of vehicle (if gavage): 20 ml/kg bw

- Justification for choice of vehicle: test substance was soluble in water

DOSAGE PREPARATION (if unusual): The substance was formulated in water using a rigid metal stomach probe in koaslantem

Doses:

5000 mg/kg bw

No. of animals per sex per dose:

Total : 10 animals
5000 mg/kg bw :5 males and 5 females

Control animals:

not specified

Details on study design:

Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of weighing: after 1 week and at the end of the
14 days animals were weighed individually.
- Necropsy of survivors performed: yes
- Other examinations performed: Clinical signs

Statistics:

No data available

Results and discussion

Preliminary study:

No data available

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: No mortality was observed in treated rats at 5000 mg/kg bw

Mortality:

No mortality was observed in treated rats at 5000 mg/kg bw

Clinical signs:

other: No poisoning symptoms were observed

Gross pathology:

The section of some animals killed at the end of the experiment showed no macroscopic findings.

Other findings:

No data available

Applicant's summary and conclusion

Interpretation of results:

Category 5 based on GHS criteria

Conclusions:

The lethal concentration (LD50) value for acute oral toxicity test was considered to be >5000 mg/kg bw ,when 5 male and 5 female Wistar (SPF cpB) rats were treated with Sodium 4-chloro-3-nitrobenzenesulphonate (17691-19-9) orally.

Executive summary:

Acute oral toxicity study was done in 5 male and 5 female Wistar (SPF cpB)rats using test material Sodium 4-chloro-3-nitrobenzenesulphonate(17691-19-9) following 14 days of observation period.Rats were 14 weeks old.Initial weight of male animals was 161 g and the female rats had an average initial weight of 160 g. The rats were conventionally kept in Makrolonkaefigen type III on dust-free wood granules at a room temperature of 22 +/- 1.5 degrees Celsius and 2 hours of light / dark rhythm (artificial lighting from 7 am to 7 pm CET) and a relative humidity of approx. 60 +/- 5% in groups of 5 each animals.The feed,Altromin R 1324 was given ad libitum. Water was provided ad libitum for approximately 16 hours and before until about 4 hours after application.Water was used as vehicle.The substance was formulated in water using a rigid metal stomach probe in koaslantem. Application volume (20 ml / kg body weight) to each 5

males and 5 females were given once. After 1 week and at the end of the 14 days animals were weighed individually.No mortality was observed at dose 5000 mg/kg bw.No poisoning symptoms were observed in animals.The section of some animals killed at the end of the experiment showed no macroscopic findings.Hence,LD50 value was considered to be >5000 mg/kg bw,when rats were treated withSodium 4-chloro-3-nitrobenzenesulphonate(17691-19-9) orally.