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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
17 October 2005 to 05 December 2005
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study according to the guideline under GLP
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0 and 100 mg/L
- Sampling method: taken in duplicate at 0 (before daphnids were added) and 48 hours; test substance analyses: both duplicates (0 h), pooled sample in duplicate (48 h): controls single sample analysed in duplicate
- Sample storage conditions before analysis: stored at 4 °C and analysed after the 48 hours samples were taken
Vehicle:
no
Details on test solutions:
31.1 mg of the substance was dissolved in 300 mL water and stirred during 5 minutes at room temperature just before introduction of the daphnids..
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Strain: Daphnia magna Straus
- Source: inhouse bred
- Age at study initiation: < 24 hours
- Feeding during test: none

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
250 mg/L as CaCO3
Test temperature:
20-21 °C
pH:
7.6-8.0
Dissolved oxygen:
8.7-9.0 mg/L (96-101% of saturation)
Nominal and measured concentrations:
nominal: 0 and 100 mg/L
measured: ND and 98-99% of nominal
Details on test conditions:
TEST SYSTEM
- Test vessel: 250 mL
- Type : closed with glass plate
- Material, size, headspace, fill volume: wide glass beaker containing 50 mL test solution
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water(according to guidance)
- Alkalinity: pH 7.8 ± 0.2
- Ca/Mg ratio: 4/1
- Na/K ratio: 10/1
- Culture medium different from test medium: similar

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16/8
- Light intensity: 570-740 Lux

EFFECT PARAMETERS MEASURED : immobilization

TEST CONCENTRATIONS
- Test concentrations: 0, 100 mg/L
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Test substance containing solutions were dark blue coloured.
Results with reference substance (positive control):
EC50 Potassium dichromate 0.53 mg/L
Reported statistics and error estimates:
NA
Validity criteria fulfilled:
yes
Conclusions:
The EC50 of the substance is > 100 mg/L
Executive summary:

In a test according to OECD 202 daphnids were exposed to 0 and 100 mg/L during 48 hours in a static test. No mortality or effects on mobility were found. The measured test concentrations were 98 -99% of nominal. The EC50 is > 100 mg/L.

Description of key information

In a test according to OECD 202 daphnids were exposed to 0 and 100 mg/L during 48 hours in a static test. No mortality or effects on mobility were found. The measured test concentrations were 98 -99% of nominal. The EC50 is > 100 mg/L.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information